复方ANBP对小鼠皮肤移植创面愈合的疗效观察

目的观察复方ANBP由仙鹤草(Agrimonia eupatoria, A),藕节(Nelumbo nucifera Gaertn, N),乳香(Boswellia carteri, B)和蒲黄(Pollen Typhae, P)组成]对皮肤移植创面愈合的促进作用。方法选用40只雌性C57BL/6小鼠,制成背部直径1.2 cm圆形全层皮肤缺损小鼠模型,后进行自体原位皮肤移植术,随机分为对照组和实验组,实验组给予复方ANBP粉末敷于皮肤移植区,对照组不予处理。通过肉眼、HE染色观察比较两组皮肤移植区14 d及21 d时皮肤愈合情况,计算创面愈合率并进行统计学分析。结果 (1)皮肤移植14 d、21 d后,实验组小鼠创面愈合率高于对照组(P<0.05)。(2)HE染色结果:皮肤移植14 d,实验组较对照组皮肤移植交界处平坦、表皮嵴浅且连接紧密;皮肤移植区表皮层变薄、新生毛囊数量较多且发育良好。皮肤移植21 d,实验组较对照组皮肤移植交界处皮下组织紧密呈更强的收缩感;皮肤移植区毛囊更成熟、发育趋于完善,出现了明显的毛干、皮脂腺等皮肤附属器。结论复方ANBP能够加快皮肤移植区与周围皮肤衔接,加速皮肤移植愈合,促进皮肤附属器发育及皮肤塑形,提高愈合质量。

Therapeutic Effects of Compound ANBP on Wound Healing in Mouse Skin Grafts

Objective To observe the improvement of compound ANBP (Agrimonia eupatoria, A),(Nelumbo nucifera Gaertn, N),(Boswellia carteri, B),(Pollen Typhae, P)] for the wound healing of skin grafting. Methods Forty female C57 BL/6 mice were used to create a 1.2 cm round full-thickness skin defect wound on the back of each mouse. Autologous skin transplantation was performed and randomly divided into a control group and an experimental group. The experimental group was given compound ANBP powder applied to the skin grafting area and the control group was not treated. The skin tissue healing of the 2 groups of skin grafting at 14 d and 21 d was observed and compared by naked eye and HE staining, and the wound healing rate was calculated and statistical analysis was performed. Results(1) After 14 d and 21 d of skin grating, the wound healing rate of the experimental group was higher than the control group(P<0.05).(2) HE staining results showed that at14 d of skin grafting, the experimental group had a flat skin at the junction of the skin graft, and the epidermis was shallow and tightly connected;the epidermal layer of the skin graft area became thinner, and the number of new hair follicles was larger and the development was good. At 21 d of skin grating, the experimental group had a stronger contraction of the subcutaneous tissue at the skin graft junction than the control group;the hair follicle in the skin graft area was more mature and developed, and there was obvious hair shaft structure and sebaceous gland. Conclusion Compound ANBP can accelerate the connection between the skin graft area and the surrounding skin, accelerate the healing of skin grafts, promote the development of skin appendages and skin shaping, and improve the quality of healing.