羧甲基-β-环糊精为手性选择剂拆分盐酸尼卡地平等6种药物对映体

目的建立高效毛细管电泳法分离盐酸尼卡地平等6种药物对映体。方法采用未涂层熔融石英毛细管柱,30 mmol.L-1磷酸盐缓冲液作为背景电解质,手性选择剂为羧甲基-β-环糊精(CM-β-CD)。结果在最佳条件下,盐酸尼卡地平、盐酸曲马多、酮康唑、氧氟沙星、阿替洛尔、盐酸特布他林的分离度(R)分别为3.44、1.50、2.37、7.50、1.83和2.80。结论 CM-β-CD对6种药物对映体均有较好的对映体选择性。该分离方法操作简单,重现性好。

Enantiomeric separation of six drugs by high performance capillary electrophoresis

Objective To establish a method of high performance capillary electrophoresis for the chiral separations of six drugs. Methods The separations of enantiomers were performed in an untreated fused-silica capillary tube, using CM-β-CD as chiral selectors, buffer of 30 mmol·L^-1 NaH₂PO₄. Results Under the optimal conditions, the resolutions of nicardipine hydrochloride, tramadol hydrochloride,ketoconazole,ofloxacin,atenolol and terbutaline hydrochloride were 3.44, 1.50, 2.37, 7.50, 1.83 and 2.80, respectively. Conclusion CM-β-CD is highly enantioselective for the six drugs . The method is simple, fast with an improved reproducibility.