| 腺苷受体激动剂对大鼠脊髓损伤后细胞外液兴奋性氨基酸的影响 |
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| 引用本文： | 张秋林,李明,赵杰,叶晓健,侯铁胜,张玲妹. 腺苷受体激动剂对大鼠脊髓损伤后细胞外液兴奋性氨基酸的影响[J]. 第二军医大学学报, 2000, 21(1): 71-73 |
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| 作者姓名： | 张秋林 李明 赵杰 叶晓健 侯铁胜 张玲妹 |
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| 作者单位： | 1. 第二军医大学长海医院骨科,上海,200433
2. 上海医科大学神经生物国家重点实验室 |
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| 基金项目： | 上海市科委带头人基金!资助项目（97XD14015） |
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| 摘 要： | 目的：观察腺苷对脊髓损伤后细胞外液兴奋性氨基酸（ＥＡＡ）的影响,研究腺苷脊髓损伤后的神经保护机制。方法：腹侧压迫损伤法造成大鼠脊髓Ｔ１３中度损伤,用微透析法收集伤前１５ｍｉｎ和伤后即刻,１５,３０,４５ｍｉｎ脊髓Ｔ１３的细胞外液。治疗组伤后立即灌注非特异笥腺苷受体激动剂２－氯腺苷（２－ＣＡＤＯ,０．１ｍｍｏｌ／Ｌ）。采用高效液相色谱仪荧光检测法检测透析液中谷氨酸和天门冬氨酸含量。
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| 关 键 词： | 腺苷 氨基酸 脊髓损伤 2－CADO 大鼠 |
| 文章编号： | 0258-879X(2000)01-0071-03 |
| 修稿时间： | 1999-09-13 |
Effect of adenosine agonist on extracellular excitatory amino acid after spinal cord injury in rats |
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| ZHANG Qiu-Lin,LI Ming,ZHAO Jie,YE Xiao-Jian,HOU Tie-Sheng,ZHANG Ling-Mei. Effect of adenosine agonist on extracellular excitatory amino acid after spinal cord injury in rats[J]. Former Academic Journal of Second Military Medical University, 2000, 21(1): 71-73 |
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| Authors: | ZHANG Qiu-Lin LI Ming ZHAO Jie YE Xiao-Jian HOU Tie-Sheng ZHANG Ling-Mei |
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| Affiliation: | ZHANG Qiu-Lin(Department of Orthopedics, Changhai Hospital, Second Military Medical University,Shanghai 200433, China)LI Ming(Department of Orthopedics, Changhai Hospital, Second Military Medical University,Shanghai 200433, China)ZHAO Jie(Department of Orthopedics, Changhai Hospital, Second Military Medical University,Shanghai 200433, China)YE Xiao-Jian(Department of Orthopedics, Changhai Hospital, Second Military Medical University,Shanghai 200433, China)HOU Tie-Sheng(Department of Orthopedics, Changhai Hospital, Second Military Medical University,Shanghai 200433, China)ZHANG Ling-Mei(Neurobiology National Major Laboratory, Shanghai Medical University) |
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| Abstract: | Objective: To observe the effect of adenosine on extracellular excitatory amino acid (EAA) after spinal cord injury (SCI) so as to explore its neuroprotective mechanism on SCI. Methods: Moderate spinal cord injury of T 13 in rats were abtained by using ventral compression injury model. The extracellular fluids were collected 15 min before injury and after every 15 min till 60 min after injury by using microdialysis. Glutamate and asparate in the samples were analyzed by an HPLC. In treatment group, 2 chloroadenesine (2 CADO,0.1 mmol/L), one of nonspecific adenosine agonists, was given through the probe immediately after injury. Results: The concentration of glutamate and asparate in extracellular fluid increased by 5 and 7 times respectively during the first 15 min after SCI, and decreased to pre injury level 45 min after SCI. Conclusion: Treatment with 2 CADO can inhibit the increase of glutamate and asparate significantly ( P <0 01). Adenosine plays a neuroprotective role partly through inhibiting excitotoxicity of EAA. |
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| Keywords: | adenosine amino acid excitotary spinal cord injuries |
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