2008年-2010年湖州市手足口病病原监测分析

目的:了解湖州市手足口病的感染情况,为制定预防和控制措施提供科学依据。方法:采用荧光定量RT-PCR方法,对2008年-2010年湖州市765份疑似手足口病感染者的疱疹液、咽拭子及粪便标本进行肠道病毒、肠道病毒71型和柯萨奇病毒A16型的核酸检测。结果:肠道病毒通用核酸阳性510例,阳性率为66.67%(510/765)。其中EV71阳性228例,阳性率为29.80%(228/765);Cox A16阳性134例,阳性率为17.52%(134/765);其他EV病毒阳性148例,阳性率为19.35%(148/765)。男女阳性率分别为67.46%(313/464)和65.44%(197/301)。5岁以下年龄组儿童阳性率为67.64%,是感染的主要人群。结论:EV71是引起湖州市儿童手足口病的主要病原,不同性别间EV、EV71及Cox A16的发病率无显著性差异。定期开展手足口病病原学监测,将有助于提出更好的预防和控制措施。

Pathogen surveillance on hand-foot-mouth disease in Huzhou during 2008-2010

Objective:To investigate the major etiological condition of hand-foot-mouth disease(HFMD) in Huzhou.Methods:A total of 765 specimens including herpes fluid,throat swab and stool were collected from clinically diagnosed cases with HFMD,and the viral nucleic acids of enterovirus(EV),enterovirus 71(EV71) and Coxsackievirus A16(CoxA16) were tested by fluorescence quantitative RT-PCR.Results:510 of 765 specimens were positive for EV by real-time RT-PCR,the positive rate was 66.67%(510/765).Among them,228 specimens were identified as EV71 infection,the positive rate was 29.80%(228/765);134 cases were CoxA16 infection,the positive rate was 17.52%(134/765);the other 148 specimens belonged to other enterovirus infection,the positive rate was 19.35%(148/765).The positive rates for men and women were 67.46%(313/464) and 65.44%(197/301) respectively.The EV positive rate was 67.64% in children under 5 years,which was the high-risk group of EV infection.Conclusion:EV71 was considered to be the major cause of HFMD in children in Huzhou,the incidence of EV,EV71 and Cox A16 infection among male and female has non-significant difference.Regular etiological surveillance on hand-foot-mouth disease would contribute to develop control and prevention measures.