成人骨髓间质干细胞定向诱导为神经元样细胞的研究

目的：体外定向诱导成人骨髓间质干细胞(MSC)分化为神经元样细胞。方法：采用Ficoll-Paque液（1.077×10³ g/L）离心分离成人MSC，体外扩增，分别采用含巯基乙醇和硫代甘油等试剂的无血清DMEM诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶（NSE）、神经丝蛋白（NF）、胶质纤维酸性蛋白（GFAP）的表达。结果：成人骨髓间质干细胞在体外扩增5代可获5×10⁷个细胞。加入巯基乙醇等或硫代甘油等诱导剂诱导后，MSC胞体收缩，突起伸出；免疫组化显示诱导出的神经元样细胞NSE、NF表达阳性，GFAP阴性。结论：成人骨髓间质干细胞在体外可以分化为神经元样细胞。

Human mesenchymal stem cells differentiate into neuron-like cells

AIM:To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells.METHODS:hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate with DMEM/monothioglycerol or DMEM/β-mercaptoethanol, respectively. Neuron-specific enolase(NSE), neurofilament(NF), and glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry. RESULTS:hMSC were expanded as undifferentiated cells in culture for more than 5 passages. When treated with monothioglycerol or β-mercaptoethanol for 5 hours, hMSC exhibited neuronal phenotype. The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION:hMSC can be induced to differentiate into neurous.