黄芩苷对SH-SY5Y细胞损伤的Bcl-2和Bcl-xL mRNA基因表达的影响

目的 探讨黄芩苷对过氧化氢(H₂O₂)诱导的SH-SY5Y细胞损伤的Bcl-2和Bcl-xL mRNA表达的影响. 方法 建立人神经母细胞瘤SH-SY5Y细胞的体外H₂O₂损伤模型,采用噻唑蓝(MTT)法检测不同浓度黄芩苷对SH-SY5Y细胞存活率的影响,采用Real-time PCR法检测各组细胞的Bcl-2和Bcl-xL表达水平的变化. 结果 50,100,200 μmol.L^-1黄芩苷组细胞存活率分别为130.4%,89.9%和60.9%,H₂O₂损伤组细胞存活率为33.9%,50,100 μmol.L^-1黄芩苷组与H₂O₂损伤组比较,差异有统计学意义(P<0.01). 50,100,200 μmol.L^-1黄芩苷组Bcl-2 mRNA表达量分别为(11.48±0.48),(7.37±1.57),(7.39±2.01),H₂O₂损伤组为(5.84±0.58);50,100,200 μmol.L-1黄芩苷组Bcl-xL mRNA表达量分别为(19.96±2.22),(11.36±3.94),(13.07±2.37),H₂O₂损伤组为(7.95±0.58),50 μmol.L^-1组Bcl-2 和Bcl-xL mRNA与H^₂O^₂损伤组比较,差异均有统计学意义(均P<0.05). 结论 黄芩苷对H₂O₂诱导的SH-SY5Y细胞损伤的保护作用,可能与黄芩苷上调Bcl-2和Bcl-xL的表达而起到抗凋亡的作用有关.

Effects of Baicalin on Bcl-2 and Bcl-xL mRNA Expression Against SH-SY5Y Cells Apoptosis

Objective To study the effects of baicalin on H2O2-induced SH-SY5Y cell injury and Bcl-2 and Bcl-xL mRNA expression.Methods The H2O2-induced SH-SY5Y cell injury was established on SH-SY5Y neuroblastoma cell line in vitro.MTT assay was used to detect the proliferation of SH-SY5Y cells under different concentrations of baicalin,and Bcl-2 and Bcl-xL mRNA expression were measured by Real-time quantitative PCR.Results The survival rates of cells were 130.4%,89.9% and 60.9% by baicalin at 50,100,200 μmol·L-1,respectively,the two low dose of which showed significantly difference compared with the H2O2 injury model group which was 33.9%(P<0.01).Bcl-2 mRNA expression levels were(11.48±0.48),(7.37±1.57),(7.39±2.01),and(5.84±0.58);Bcl-xL mRNA expressions were(19.96±2.22),(11.36±3.94),(13.07±2.37),and(7.95±0.58)in the group of 50,100,200 μmol·L-1 of baicalin and H2O2 injury control one,respectively.The conspicuous difference was found on Bcl-2 and Bcl-xL mRNA expression between the H2O2 injury group and baicalin at 50 μmol·L-1(P<0.01).Conclusion Baicalin can protect SH-SY5Y cell from H2O2 injury,the mechanism of which may be associated with Bcl-2 and Bcl-xL up-regulation resulting in anti-apoptosis effects.