实验脾虚证胃窦及十二指肠粘膜G、D细胞的变化及意义

目的：探讨Ｇ细胞（分泌胃泌素）、Ｄ细胞（分泌生长抑素）与脾虚证发生的关系。方法：应用抗－胃泌素、抗－生长抑素的多克隆抗体和免疫组织化学技术标记胃窦、十二指肠粘膜Ｇ、Ｄ细胞，运用医学图像分析系统（ＭＩＰＳ）对Ｇ、Ｄ细胞进行定量分析，并与四君子汤组对照。结果：脾虚时Ｇ、Ｄ细胞数均减少，Ｄ细胞面积缩小，Ｇ细胞灰度值增高，Ｇ／Ｄ细胞数和细胞面积比值均增高。经四君子汤预防和治疗的脾虚大鼠，Ｇ、Ｄ细胞数有所增加，Ｄ细胞面积明显增大，Ｇ细胞灰度值、Ｇ／Ｄ细胞数和细胞面积比值均接近或略低于正常。脾虚自然恢复大鼠也有一定程度的改善，但与脾虚四君子汤预防和治疗大鼠相比有显著性差异。结论：研究结果从形态学上揭示Ｄ细胞分泌ＳＳＴ亢进、Ｇ细胞释放Ｇａｓ不足、Ｇ／Ｄ细胞比例失调，是导致脾虚证胃肠功能障碍的一个重要病理机制。

Variations of G Cells and D Cells in Experimental Spleen Asthenia and Its Significance

Objective:To explore the relationship between G cells (gastrin cells), D cells (somatostatin cells)and pathogenesis of spleen asthenia syndrome. Methods:G cells and D cells in gastric and duodenal mucosa were analyzed with polyclonal antibody of Gas and SST and by immunohistochemical technique and Quantitative image analysis system (MIPS).Shijunzi decoction(SJZD) was chosen for comparison.Results:The study demonstrated that the number of G cells and D cells declined and even area of D cells declined with G cells evaluated and ratio of the number and area of G/D increased in spleen asthenia syndrome.Those pathologic changes were improved and even better than spontaneous recovery group after prevention and therapy with SJZD. Conclusions:The study confirmed that variations of G,D cells and G/D would lead to gastrointestinal disfunction,which was the important cause of pathogenesis of spleen asthenia syndrome.