铬盐职业接触对T细胞部分免疫功能的影响

目的 了解铬盐对职业接触者体内免疫指标变化的影响.方法 115名不同浓度铬盐接触工人为接触组,以远离铬盐污染区的90名居民为对照组.采用原子吸收分光光度法测定研究对象作业环境铬盐浓度;采用荧光标记流式细胞分析法测定研究对象外周血中T细胞CD3~+、CD3~+CD4~+、CD3~+CD8~+及CD4~+/CD8~+水平.结果 接触组空气中铬盐个体环境暴露浓度为(27.51±33.25)μg/m~3,对照组浓度为(0.16±0.15)μg/m~3,两组比较差异有统计学意义(z=8.045,P<0.01).铬盐接触组人群CD3~+、CD3~+CD4~+、CD3~+CD8~+及CD4~+/CD8~+平均水平分别为:(30.08±17.75)%、(1.04±1.73)%、(11.94±9.78)%、0.10±0.14;对照组为:(63.00±13.57)%、(30.51±5.16)%、(14.82±4.59)%、2.17±0.53.两组比较,对照组高于接触组,差异有统计学意义(z值分别为:4.484、5.227、1.976、-5.218,P值均<0.05).结论在铬盐个体环境暴露评价基础上,铬盐接触者以T细胞为主的细胞免疫功能受到抑制,而CD3~+CD4~+有望作为铬盐接触免疫损伤的早期效应性生物标志物,值得进行更深入研究.

The effects of occupational soluble chromate exposure on immunological function of T-cell

ObjectiveTo investigate the early changes of some immunological function of T-cell in chromate workers. MethodsA total of 115 workers exposed to different levels of soluble chromate were enrolled in exposed group; while 90 non-exposure workers who lived far away from the chromate plant were enrolled as control. The air concentration of soluble chromate was determined by atomic absorption spectrometry. CD3~+ , CD3~+ CD4~+ , CD3~+ CD8~+ and CD4~+/CD8~+ of T-cell were determined by flow eytometry analysis. ResultsThe individual air chromate concentration in the exposed group was (27.51 ±33.25)μg/m~3 ,and the control group was (0. 16±0. 15)μg/m~3. The significant difference between the two groups was observed (z = 8. 045, P < 0. 01 ). The levels of the lymphocyte subsets ( CD3~+ , CD3~+ CD4~+,CD3~+ CD8~+ and CD4~+/CD8~+) in exposed group were (30.08 ±17.75)% ,(1.04 ±1.73)% ,(11.94 ±9. 78)% ,0. 10 ± 0. 14. While, those of control group were ( 63.00±13. 57 )%, ( 30. 51±5.16 )%,( 14. 82±4. 59)% ,2. 17± 0. 53, higher than that of the exposed group (z values were 4. 484,5. 227,1. 976,-5. 218,respectively, P < 0. 05 ). ConclusionOn the basis of individual air monitoring, the cellular immune function affected by soluble chromate is mainly based on T lymphocyte inhibition. The indicators CD3~+ CD4~+ mentioned above may be considered as efficient biomarkers in further research.