红松松针中黄酮类成分的分离与鉴定

目的研究红松松针中的黄酮类成分,为松属植物的化学分类学研究提供依据。方法采用反复硅胶、聚酰胺、ODS、Sephadex LH-20柱色谱等方法进行分离纯化,根据理化性质和1H-NMR、13C-NMR等技术对分离得到的化合物进行结构鉴定。结果从红松松针中分离得到7个化合物,分别鉴定为蛇葡萄素4′-O-β-D-吡喃葡萄糖苷ampelopsin 4′-O-β-D-glucopyranoside,1]、槲皮素3-O-α-L-呋喃阿拉伯糖苷quercetin3-O-α-L-arabinofuranoside,2]、山柰酚3-O-α-L-呋喃阿拉伯糖苷kaempferol3-O-α-L-arabinofuranoside,3]、山柰酚3-O-β-D-吡喃葡萄糖苷kaempferol3-O-β-D-glu-copyranoside,4]、5,7,8,4′-四羟基-3-甲氧基-6-甲基黄酮8-O-β-D-吡喃葡萄糖苷5,7,8,4′-tetra-hydroxy-3-methoxy-6-methylfavone8-O-β-D-glucopyranoside,5]、山柰酚3-O-(5″-O-反式-阿魏酰基)-α-L-呋喃阿拉伯糖苷kaempferol3-O-(5″-O-E-feruloyl)-α-L-arabinofuranoside,6]、山柰酚3-O-(5″-O-反式-对-香豆酰基)-α-L-呋喃阿拉伯糖苷kaempferol3-O-(5″-O-E-p-coumaroyl)-α-L-arabi-nofuranoside,7]。结论化合物1-3为首次从松属植物中分离得到,4-7为首次从该种植物中分离得到。

Isolation and identification of flavonoids from pine needle of Pinus koraiensis Sieb. et Zucc.

Objective To study the flavonoids of pine needles of Pinus koraiensis Sieb. et Zucc. Methods Compounds were isolated by the silica gel, ODS, Sephadex LH-20 columns, and their structures were identified by the physicochemical characters and spectral analysis (¹H-NMR, ¹³C-NMR). Results Five compounds were obtained and identified as ampelopsin 4′-O-β-D-glucopyranoside (1), quercetin 3-O-α-L-arabinofuranoside (2), kaempferol 3-O-α-L-arabinofuranoside (3), kaempferol 3-O-β-D-glucopyranoside (4), 5,7,8,4′-tetrahydroxy-3-methoxy-6-methylfavone 8-O-β-D- glucopyranoside (5). Conclusions Compounds 1-3 are obtained from Pinus Linn. for the first time, compounds 4-5 are obtained from this species for the first time.