STUDY ON THE HETEROGENEITY OF TSH RECEPTOR ANTIBODIES IN THE PATHOGENESIS OF AUTOIMMUNE THYROID DISEASE

ffeSllm6 Objectif Pour studier l' heterogdneite des anticorps du rdcepteur 7SH chez ies ~lades AITD (autoimmunethyroid disease). ANthaes Six malades aunt des anticorps heterogdnes du rdcepteur TSH out ate selectional. Chez cesmalades out did dtablis des clones des cellules B hiV-transfonha. theultats to valeur de TBll (124TSH binding inhibitingimmunoglobulin ) vane de 51 % d 89% chez ces malades. gb (thyroid stimulating anti~) existe chef ies malades M 1,2,4, 5 et 6 alors que seuls ies…

STUDY ON THE HETEROGENEITY OF TSH RECEPTOR ANTIBODIES IN THE PATHOGENESIS OF AUTOIMMUNE THYROID DISEASE

Objective To investigate the heterogeneity of TSH receptor antibodies ofpatients with autoimmune thyroid disease (AITD). Methods Six patients with heterogeneous TSH receptor antibodies were selected. The EBV-transformed B cell clones producing monoclonal anti-thyrotropin receptor antibodies were established from those selected patients. Results 125I-TSHbinding inhibiting immunoglobulin (TBII) values of the 6 patients ranged from 51 % to 89%. Thyroid stimulating antibody (TSAb) existed in patients No. 1,2, 4, 5 and 6 while only patients No. 1,2 and 3 had thyroid stimulation-blocking antibody (TSBAb ). After EBV-transformation of the peripheral B lymphocytes from those patients, we isolated and characterized 5 Bcell clones prepucing the TBII from patient No. 3 and 4 B cell clones prepucing TSAb from patient No. 6. Conclusion Autoantibodies against the us receptor are polyclonal and consist of heterogeneous populations with both stimulatory and inhibitory effects.