伸筋易骨法调控软骨细胞内钙离子浓度干预细胞代谢的机制研究

目的]分析伸筋易骨法对L型钙通道活性及软骨细胞内钙离子浓度影响探讨伸筋易骨法防治膝骨性关节炎(KOA)的力学-生物转导机制。方法]将50只日本大耳白兔编号并随机分成5组,观察组、对照组、模型组、空白组、假手术组各10只,采用改良的Hulth造模法模拟应力失常诱导的KOA模型,观察组以伸筋易骨法治疗,对照组给予关节腔注射玻璃酸钠注射液治疗。通过激光共聚焦显微镜检测各组原代软骨细胞内钙离子浓度,运用膜片钳技术检测各组软骨细胞L型钙通道电流密度。结果]观察组、对照组原代软骨细胞内钙离子浓度均高于模型组,差异具有统计学意义(P0.05);观察组、对照组软骨细胞内L型钙通道电流密度均高于模型组,差异具有统计学意义(P0.05)。结论]伸筋易骨法上调细胞表面L型钙通道活性,增加软骨细胞内钙离子浓度,进而发挥促软骨细胞合成代谢的作用,该机制可能是推拿手法或同类物理治疗发挥作用的重要原因之一。

Mechanisms study of Shenjin Yigu method regulated calcium ion concentration in chondrocyte to intervene cell metabolism

Objective] To analyze the effect of Shenjin Yigu method on L-type calcium channel activity and intrachondrocyte calcium concentration,and to explore the mechanism of mechanical-biological transduction of Shenjin Yigu method in the prevention and treatment of knee osteoarthritis (KOA).Methods] Fifty Japanese white rabbits were randomly divided into five groups. The KOA model induced by stress disorder was simulated by modified Hulth model. The observation group was treated with Shenjin Yigu method,while the control group was treated with sodium hyaluronate injection into articular cavity. Calcium ion concentration in primary chondrocytes of each group was measured by confocal laser microscopy,and current density of L-type calcium channel in chondrocytes of each group was measured by patch clamp technique.Results] Calcium concentration in primary chondrocyte of observation group and control group was higher than that of model group,with statistical significance (P<0.05). Tthe current density of L-type calcium channel in observation group and control group was higher than that of model group,with statistical significance (P<0.05).Conclusion] Shenjin Yigu method can regulate the activity of L-type calcium channel on the surface of chondrocyte,increase the concentration of calcium ion in chondrocyte,and then promote the synthesis and metabolism of chondrocyte. This mechanism may be one of the important reasons for the effect of massage manipulation or similar physiotherapy.