3cm以下直肠癌细胞DNA倍性流式细胞术分析及其临床意义

应用流式细胞术测定了51例直径3cm以下直肠癌石蜡包埋瘤组织细胞DNA倍性。结果表明,Dukes A,B,C三期肿瘤二倍体发生率分别为83％、52％及21％。各期间二倍体瘤发生率差异有非常显著性(P<0.01)。非二倍体组直肠癌淋巴结转移阳性率明显高于二倍体癌组(P<0.01)。DNA倍体与组织学分级关系不明显(P>0.05)。二倍体癌病人术后5年及累计生存率均非常显著地高于非二倍体癌者(分别P<0.01)。本组结果提示,DNA倍性分析可为3cm以下直肠癌提供新的预后参数,并对其治疗可能具有较好的指导意义。

Flow Cytometric Analysis of Nuclear Ploidy in Rectal Cancer Less Than 3cm in Diameter

Nuclear ploidy of paraffin-embedded tumor tissues of 51 rectal cancer of less than 3cm in diameter was measured by flowcytometry.Relationship of chromosome ploidy level to Dukes stage,histological grade,lymph node metastases and survival rate of patients was analysed. The results showed that the incidence of diploid in petients with Dukes stage A,B,C tumors was respectively 83%, 52% and 21% with significant differences.There were remarkably more patients with lymph node metastases in nondiploid cancer group than in diploid cancer group(P<0. 01). The pa- tients with diploid cancer had a significantly better 5-year and cumulative survival rates than those with aneuploid can- cers(P<0. 01 ).This study suggested that analysis of cellular chromosome ploidy could provide a new prognostic param- eter for rectal cancer less than 3cm in diameter. It plays an important role in options of treatment.