人类LAK细胞体外实验性扩增及生物学活性分析

由癌症患者外周血中分离出少量ＬＡＫ前体细胞。在ＬＡＫ细胞培养体系中加入重组人类白细胞介素２，植物血球凝集素及辅助细胞，明显提高了ＬＡＫ细胞的体外增殖能力并延长了ＬＡＫ细胞的存活时间。表型分析发现，ＬＡＫ－Ｌ细胞的ＩＬ－２受体（ＩＬ－２－Ｒ－）表达水平明显高于ＬＡＫ－Ｓ细胞，ＬＡＫ－Ｌ细胞的体外大量增殖具有以ＯｋＴ＾＋ ８表型为主的单亚群增殖特性。体外杀伤实验及裸小鼠体内成瘤抑制实验结果表明，用此法

Researches of Antitumor Effect of LAK Cells from Cancer Patient Reproduced by Long Term Culture in vitro

To augment the in vitro reproduction of LAK cells,we add highly purified human recombinant in- terleukin-2(rIL-2)with phytohemagglutinin(PHA)and helper cells to the culture system,and,as a result,huge number of LAK cells (LAK-L)were harvested from the initial limited number of LAK cell obtained from the cancer patient's pe- ripheral blood. These LAK-L cells exhibited similar powerful antitumor activities both in vitro and in vivo as compared with LAK-S cell(LAK cells activated in short term culture), The phenotyping results suggestod that there were more ex- pression of IL-2 receptors in LAK-L cell than did the LAK-S cells. The rapid proliferation of LAK-L cells was character- ized by single subgroup(OKT_8~ cells)expansion of the LAK-L populations.