Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium |
| |
引用本文: | 屈岭,梁晓春,张宏,吴群励,孙连庆,顾蓓.Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium[J].Chinese Journal of Integrated Traditional and Western Medicine,2008,14(4):293-297. |
| |
作者姓名: | 屈岭 梁晓春 张宏 吴群励 孙连庆 顾蓓 |
| |
作者单位: | Department of Traditional Chinese Medicine,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Cell Center,Institute of Basic MedicalScience,Peking Union Medical College,Chinese Academy of Medical Sciences,Cell Center,Institute of Basic MedicaIScience,Peking Union Medical College,Chinese Academy of Medical Sciences |
| |
摘 要: | Objective:To investigate the effect of Jinmaitong(筋脉通,JMT)serum on the proliferation of rat Schwann cells(SCs)primarily cultured in high glucose medium.Method:SOs were primarily cultured in Dulbecco's minmum essential medium(DMEM control),50 mmol/L glucose medium(50 mmol/L Glu),75 mmol/L glucose medium(75 mmol/L Glu),as well as 50 mmol/L glucose medium,with different concentrations of JMT serum(undiluted,1:2 diluted and 1:8 diluted)and Neurotropin(Ntp), respectively.The proliferation of SCs under different conditions was detected by MTT.Result:SCs grew exuberantly in DMEM within 24-72 h,but slowed down at 96 h.The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48,72 and 96 h,which showed that both were significantly different compared to the control group(P<0.01).The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu(P<0.05).Spearman's rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose(r=-0.471,P<0.01).Excluding the time factor,partial correlation showed similar results(r=-0.679,P<0.01).After 48 h,the proliferation of SCs increased significantly in JMT1:2 and Ntp compared with 50 mmol/L Glu(control 0.437±0.019,50 mmol/ L Glu 0.367±0.035,JMT1:2 0.426±0.024,Ntp 0.422±0.013;P<0.01),and there were no statistically significant differences among the JMT groups,the Ntp group and the control group(P>0.05).Conclusions: The proliferation of SCs was inhibited in high glucose medium,and the inhibition was reduced by different concentrations of JMT serum,especially at JMT1:2.
|
收稿时间: | 29 April 2009 |
Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium |
| |
Authors: | Ling Qu Xiao-chun Liang Hong Zhang Qun-li Wu Lian-qing Sun and Bei Gu |
| |
Institution: | (1) Institute of Anatomy and Cell Biology, University of Freiburg, Albertstrasse 23, 79104 Freiburg, Germany;(2) Department of Neurosurgery, University Hospital Freiburg, Breisacher Strasse 64, 79106 Freiburg, Germany;(3) Institute of Anatomy and Cell Biology, P.O. Box 111, 79001 Freiburg, Germany; |
| |
Abstract: | Objective To investigate the effect of Jinmaitong (筋脉通, JMT) serum on the proliferation of rat Schwann cells (SCs) primarily cultured
in high glucose medium. |
| |
Keywords: | Schwann cells high glucose primary culture Jinmaitong proliferation Chinese herbs' serum pharmacology |
本文献已被 CNKI 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《Chinese Journal of Integrated Traditional and Western Medicine》浏览原始摘要信息 |
| 点击此处可从《Chinese Journal of Integrated Traditional and Western Medicine》下载免费的PDF全文 |
|