| Preliminary study on
cryopreservation of intact mouse ovaries by vitrification using ethylene glycol as
cryoprotectant |
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| 作者姓名： | " target="_blank">Zhang Jiarong Wan Xiaoping " target="_blank">Wan Xiaoping Li Shuangdi" target="_blank">Li Shuangdi Liu Ling" target="_blank">Liu Ling Yang Yixia" target="_blank">Yang Yixia Yan Qin" target="_blank">Yan Qin |
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| 作者单位： | Zhang Jiarong（Department of Obstetrics and Gynecology,Shanghai First
People's Hospital,Shanghai Jiaotong University, Shanghai 200080, China）
Wan Xiaoping （Department of Obstetrics and Gynecology,Shanghai First
People's Hospital,Shanghai Jiaotong University, Shanghai 200080, China）
Li Shuangdi（Department of Obstetrics and Gynecology,Shanghai First
People's Hospital,Shanghai Jiaotong University, Shanghai 200080, China）
Liu Ling（Department of Obstetrics and Gynecology,Shanghai First
People's Hospital,Shanghai Jiaotong University, Shanghai 200080, China）
Yang Yixia（Department of Obstetrics and Gynecology,Shanghai First
People's Hospital,Shanghai Jiaotong University, Shanghai 200080, China）
Yan Qin（Department of Obstetrics and Gynecology,Shanghai First
People's Hospital,Shanghai Jiaotong University, Shanghai 200080, China） |
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| 基金项目： | 上海市卫生局科研基金资助课题 (0 2 4 0 18) |
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| 摘 要： | Ovariantissuebankingisadevelopingtechniqueaimedtopreservefertilityinwomenatriskforprematureovarianfailure1] .Animalstudieshavedemonstratedthatfrozen thawedovariantissuecanrestorecyclicsecretionofovariansteroidsafterautograft .Pregnancyafterfrozeno variantissueautografthasbeenreportedinrats ,miceandewes2 ] .Cryopreservationprotocolsforovariantissuecanbebroadlyclassifiedas“equilibrium”(slowfreezing)or“nonequilibrium” (ultrarapidfreezing)accordingtothecoolingratesandcryoprotectiveagentsused…
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| 关 键 词： | 形态学 组织学 低温贮藏 电子显微镜 卵巢 |
Preliminary study on cryopreservation of intact mouse ovaries by vitrification using ethylene glycol as cryoprotectant |
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| Zhang Jiarong,Wan Xiaoping,Li Shuangdi,Liu Ling,Yang Yixia,YAN Qin.Preliminary study on
cryopreservation of intact mouse ovaries by vitrification using ethylene glycol as
cryoprotectant[J].Current Advances In Obstetrics and Gynecology,2004,13(6):477-480. |
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| Authors: | Zhang Jiarong Wan Xiaoping Li Shuangdi Liu Ling Yang Yixia YAN Qin |
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| Abstract: | Objective: Ultrarapid freezing method (vitrification) is an alternative method for cryopreserving ovarian tissue. The feasibility of intact murine ovaries cryopreservation was studied by vitrification. Methods: Intact mouse ovaries were cryopreserved using vitrification method and slow freezing method. The morphological alterations after frozen-thawed procedure and the apoptosis rates of primordial follicles were evaluated by histological examniation and TUNEL technique, and transmission electron microscopy (TEM) was used to observe the ultrastructural changes. Results:The percentages of normal primordial follicles in frozen-thawed groups were significantly lower than that in the control group ( P＜0.001), while no statistical difference was found between vitrification group and slow freezing group (78.37%±4.34% vs 78.82%±3.13%). Moreover, the distribution of abnormalities (nucleus, cytoplasm, and nucleus and cytoplasm) was similar (P＞0.05) in these two groups. In the vitrification group, slow freezing group and the fresh control group, the percentages of the TUNEL-positive follicles were 16.37%, 13.29% and 21.36%, respeetively. The apoptosis rates in the two cryopreservation groups showed no significant difference ( P ＞ 0.05). After the freezing-thawing procedure, subeellular structure was well preserved in both freezing groups with smililar ultrastructural changes. Conclusion: Vitrification is a convenient and efficient approach for small ovarian tissue cryopreservation. |
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| Keywords: | Cryopreservation Primordial follicle Ovary Apoptosis Mouse |
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