LPS促进磨屑诱导分泌溶骨性因子对人工关节松动的影响

目的探讨脂多糖(LPS)促进磨屑诱导分泌溶骨性因子 M-CSF、IL-1在人工关节松动中的作用.方法采集30例人体外周血,分离单核细胞(MOs),分组培养,A组(对照组):仅 MOs;B组:MOs+超高分子聚乙烯微粒(UHMWPE,未去除LPS);C组:MOs+LPS;D组:MOs+UHMWPE (去除LPS);E组:MOs+UHMWPE(去除LPS)+LPS.培养48h后,放免法测定上清中巨噬细胞集落刺激因子(M-CSF)、白介素1(IL-1)的含量.结果 E组 M-CSF、IL-1含量明显高于A、B、C、D组(P<0.05);B组M-CSF、IL-2较D组增高(P<0.05).结论 LPS具有促进UHMWPE刺激M-CSF、IL-1分泌的作用,增强了UHMWPE的溶骨效应.

Effect of LPS on osteolysis induced by wear particles in vitro

Objective To explore the possibility of Lipopolysaccharide(LPS) contribute to aseptic loosening of orthopedic implants. Methods Peripheral blood samples were obtained from healthy human donors.Monocytes(MOs) were isolated and assigned to 5 groups.Group A was treated with MOs alone,group B with MOs + ultrahigh molecular weight polylethylene(UHMWPE) debris,group C with MOs + LPS(100ng/ ml),group D with MOs + UHMWPE(LPS removal),group E with MOs + U HMWPE(LPS removal) + LPS(100ng/ ml).The cells were co-cultured for 48 hours and the mixtures were tested for osteolytic factors includingM-CSF IL-1. Results The levels of these factors were much higher in group E than in the other 4 groups.There was significant difference between group B and group D(P<0.05). Conclusions LPS can stimulate MOs to secrete osteoclast-activating cytokines,LPS and UHMWPE cooperative interact to enhance the biological effects of aseptic loosening.