睾丸孤核受体4对前列腺癌PC-3细胞依托泊苷化疗敏感性的影响 |
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引用本文: | 陈彼得,俞世成,丁献凡,朱世斌,王加良,张志根,李恭会.睾丸孤核受体4对前列腺癌PC-3细胞依托泊苷化疗敏感性的影响[J].全科医学临床与教育,2013,11(3):246-248,252. |
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作者姓名: | 陈彼得 俞世成 丁献凡 朱世斌 王加良 张志根 李恭会 |
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作者单位: | 陈彼得 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016); 俞世成 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016);丁献凡 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016);朱世斌 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016);王加良 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016); 张志根 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016); 李恭会 (浙江大学医学院附属邵逸夫医院泌尿外科,浙江杭州,310016); |
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基金项目: | 国家自然科学基金项目(项目编号:30973001),钱江人才计划项目(项目编号:2011R10039)浙江省公益性应用研究计划(实验动物)(项目编号:2008F80004) |
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摘 要: | 目的研究睾丸孤核受体4(TR4)基因对前列腺癌PC-3细胞依托泊苷(VP16)化疗敏感性的影响。方法观察VP16处理后PC-3细胞TR4基因的表达变化;建立TR4过表达的PC-3细胞系,利用MTT法测药物半数抑制剂量(IC50)、细胞存活率,流式细胞仪检测细胞凋亡,观察不同TR4表达水平的PC-3细胞VP16化疗敏感性的差异。结果VP16处理后,PC-3细胞TR4基因表达升高(0.42±0.02)vs(0.58±0.08);t=3.30,P〈0.05]。TR4过表达的PC-3细胞VP16IC50升高(30.83±1.32)μg/ml vs(98.89±4.21)μg/ml;F=20.38,P〈0.05]。VP16作用下,TR4过表达PC-3胞存活率较普通PC-3细胞升高,细胞凋亡则下降(0.49±0.06)vs(0.18±0.04);t=7.80,P〈0.05]。结论TR4降低前列腺癌PC-3细胞对化疗药物VP16的敏感性。
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关 键 词: | 前列腺癌 化疗 依托泊苷 睾丸孤核受体4 |
Effect of testicular orphan receptor 4 on chemo-sensitivity of etoposide in PC3 cells |
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Institution: | CHEN Bide, YU Shicheng,DING Xianfan, et al. Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016,China |
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Abstract: | Objective To study the influence of testicular orphan receptor 4 on chemo-sensitivity of etoposide in PC-3 cells. Methods TR4 expression was detected after PC-3 cells were treated with etoposide. TR4 overexpressed PC-3 cell line was established. The chemo-sensitivity of VP16 in PC-3 cells with different TR4 levels was evaluated via ICS0, cell proliferation and apoptosis. Results TR4 gene expression was increased in PC-3 cells after VP16 interference (0.42±0.02) vs (0.58±0.08);t=3.30,P〈0.05]. IC50 of VP16 was significantly higher in TR4 overexpress PC-3 cells compared with parental PC-3 cells (30.83±1.32)μg/ml vs (98.89±4.21)μg/ml;F=20.38, P〈0.05]. Cell proliferation was faster and apoptosis was less in TR4 overexpress PC-3 cells(0.49±0.06) vs (0.18±0.04) ;t=7.80, P〈0.05]. Conclusions TR4 in- hibits the chemo-sensitivity of etoposide in PC-3 cells. |
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Keywords: | prostate cancer chemotherapy etoposide testicular orphan receptor 4 |
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