Design Considerations for a Microfluidic Device to Quantify the Platelet Adhesion to Collagen at Physiological Shear Rates |
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Authors: | Durga P Sarvepalli David W Schmidtke and Matthias U Nollert |
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Institution: | (1) School of Chemical Biological and Material Engineering, University of Oklahoma Bioengineering Center, University of Oklahoma, 100 E. Boyd St. Energy Center, Norman, OK 73019, USA |
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Abstract: | Accurate assessment of blood platelet function is essential in understanding thrombus formation which plays a central role
in cardiovascular disease. Parallel plate flow chambers have been widely used as they allow for platelet adhesion on a collagen
surface at physiologically relevant fluid mechanical forces. Standard parallel plate flow chambers typically need several
milliliters of blood, which is substantially more than can be obtained from small animals. We designed, fabricated, and assessed
the functionality of a microfluidic channel with a width of 500 μm and a height of 50 μm in which a wall shear rate of 1000 s−1 can be achieved with a flow rate of 15 μL/min. The velocity distribution in the microchannel predicted from the equations
of motion was compared to experimentally measured velocities of fluorescent beads. This analysis showed that the motion of
beads was quite similar to the predicted motion. Adhesion of platelets from whole blood at a shear rate of 1000 s−1 onto a collagen surface using the microfluidic flow channel was qualitatively similar to platelet adhesion observed with
a standard sized parallel plate flow chamber. After 5 min flow the surface coverage of platelets in the microfluidic device
was about 55% while in a traditional size flow chamber the surface coverage was about 75%. This suggests that the microfluidic
flow chamber can be used to quantify platelet adhesion for system where only very small amounts of blood are available. |
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Keywords: | Microfluidics Thrombosis Platelets Shear stress |
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