环磷酰胺预处理后再免疫豚鼠听功能的变化

目的建立高阳性率自身免疫性内耳病动物模型,以供圆窗给药治疗内耳病的研究.方法白色红目豚鼠97只,其中32只用于制备内耳抗原,其余分为试验组(n=47)和对照组(n=18).试验组环磷酰胺腹腔注射进行预处理,2 d后用粗制内耳抗原行皮下多点接种,接种后4、6、8、10、12、14、20 d,接受听性脑干诱发电位(ABR)检测.对照组I不行任何处理,组Ⅱ仅行环磷酰胺预处理,不行粗制内耳抗原接种.结果接种后4 d时,试验组ABR阈值提高10 dB以上的动物占67%;接种后8 d时为86%,14 d时仍为60%,至接种后20 d时,所有动物ABR阈值恢复正常.结论将豚鼠用环磷酰胺预处理后,粗制内耳抗原只需单次接种,即可建立听力损害高发生率的自身免疫性内耳病动物模型.

Hearing Function Change of the Animals Inoculated with CIEAg-CFA after Cyclophosphamide Pretreatment

Objective To establish in a high positive way an experimental autoimmune inner ear disease model,which could be adopted for the study on drug treatment for inner ear disease via round window. Methods Ninety-seven guinea pigs were used as experimental animals,and 32 among them provided crude inner ear antigens(CIEAg).Two days after pretreatment with cyclophosphamide,the guinea pigs were inoculated intracutaneously at multiple sites with 0.2 mL of an emulsion consisting of equal parts of CIEAg and complete Freund's adjuvant(CFA).In the control group,animals received no injection or only cyclophosphamide and CFA.After 4,6,8,10,12,14,20 d of immunization,the animals were tested with ABR measurement. Results Four days after immunization,significant ABR threshold shift occurred in 67% of the experimental animals,86% at 8 d,and 60% at 14 d.(Conclusion)An ideal model of autoimmune inner ear disease for drug treatment of inner ear disease via round window can possibly be established by a single inoculation of CIEAg-CFA emulsion after pretreatment with cyclophosphamide in the guinea pig.