正常妊娠及妊高征孕妇胎盘组织中细胞凋亡及增殖基因表达的研究

目的 :探讨正常妊娠及妊高征 (PIH)孕妇胎盘组织bcl - 2、bax及ki6 7基因的表达及其相互关系。方法 :采用免疫组化方法测定正常早、中、晚期妊娠 30例及轻、中、重度PIH 36例的胎盘组织bcl - 2、bax及ki6 7的表达。结果 :(1)bcl - 2表达主要定位在绒毛的合体滋养层细胞 (S -cells) ,而bax在S -cells和细胞滋养层细胞 (C -cells)均呈阳性 ,在绒毛间质中bax也呈阳性表达 ,但强度低于滋养层细胞。ki6 7定位于C -cells;(2 )bcl - 2在晚期妊娠组的阳性率低于早、中期妊娠组 (P <0 .0 5 )。ki6 7在正常早、中、晚期妊娠组 ,对照组与PIH组 ,轻、中、重度PIH组之间均有显著性差异 (P <0 .0 1) ;(3)bcl - 2和bax表达及与ki6 7的表达无相关性 (P >0 .0 5 )。结论 :bcl - 2及bax在高水平上达到平衡 ,在胎盘组织中并不起介导细胞凋亡的主导作用 ,而且二者的表达不影响细胞的增殖

Study on expression of apoptotic and proliferative gene in placenta tissue of normal pregnancy and pregnancy induced hypertension

Objective:To investigate expression of bcl-2,bax,ki67 genes and their relations in placentas.Methods:Expressions of bcl-2,bax and ki67 genes were studied using immunohistochemical methods in 30 placentas of normal pregnancy and 36 placentas of pregnancy induced hypertension(PIH).Results:(1)bcl-2 was in syncytiotrophoblasts (S-cells).Bax was found in S-cells,cytotrophoblasts (C-cells) and villous mesenchymal cells.Ki67 was in C-cells;(2)The positive rate of bcl-2 in term pregnancy was lower than that in early and middle pregnancy(P< 0.05),Ki67 expression was significantly different between the three groups of normal pregnancy and the three groups of PIH were significantly higher in PIH than that in control group;(3)No relations were observed between bcl-2 of bax and ki67 expression. Conclusions:Expression of bcl-2 and bax seems not the crucial mediator on cell apoptosis because they are balanced at a higher level and they do not affect cell proliferation in placenta