气相色谱法测定鲨肝醇片含量

目的:建立测定鲨肝醇片含量的方法。方法:采用气相色谱法。色谱柱为PEG-20M石英毛细管柱,柱温采取程序升温,载气为高纯氮气,分流比为1∶20,检测器为氢火焰离子化检测器,进样口/检测器温度为300℃,进样量为1μl;以联苯胺为内标。另与该制剂的标准测定方法滴定法比较含量测定结果。结果:鲨肝醇检测质量浓度线性范围为1.0⁵.0μg/ml(r=0.999 4);回收率为101.2%(RSD=0.92%,n=3)。两种方法测定结果基本一致。结论:建立的方法操作简便、准确度好,可用于鲨肝醇片的含量测定。

Content Determination of Batilol Tablets by GC

OBJECTIVE： To establish a method for the content determination of Batilol tablets. MEHHODS： GC method was adopted. The determination was performed on PEG-20M quartz capillary column with temperature of 280 % by temperature pro- gramming; high purity nitrogen was used as carrier gas, and split ratio was 1 ： 20. FID was adopted with temperature of 300 %, and sample volume was 1μl. Benzidine was used as internal standard. Results by the method it were compared with those by titra- tion, RESULTS： The linear range of batilol were 1.0-5.0μg/ml （r=0.999 4） with an average recovery of 101.2% （RSD=0.92%, n = 3）. Results of 2 methods were similar. CONCLUSIONS： The method is simple, accurate and suitable for the content determina- tion of Batilol tablets.