Monoclonal antibodies against diagnostic Anisakis simplex antigens |
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Authors: | R Iglesias J Leiro M T Santamarina M L Sanmartín F M Ubeira |
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Institution: | (1) Laboratorio de Parasitología, Facultad de Farmacia Universidad de Santiago de Compostela Santiago de Compostela, Spain e-mail: mpubeira@usc.es, ES |
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Abstract: | Five monoclonal antibodies (UA2, UA3, UA5, UA6, and UA8) specific for Anisakis simplex are described. All are IgG1/κ monoclonal antibodies, except for UA2, which is an antibody IgM/κ. The molecular weights of
the major components recognized in immunoblotting are 48 and 67 kDa (UA2); 139 kDa (UA3 and UA5; same epitope); 35, 38, and
139 kDa (UA6); and 205 kDa (UA8). UA2 was the only monoclonal antibody to recognize both components of an excretion-secretion
antigen preparation and antigens in the excretory cell and esophageal glands of third-stage A.␣simplex larvae; antigens in the excretory cell were also recognized by UA3 and UA6. Cross-reactivity studies using a hyperimmune
polyclonal rabbit serum reacting with various ascaridoid nematodes indicated that the antigens captured by our monoclonal
antibodies were specific for A. simplex. Finally, comparative studies of our monoclonal antibodies and An2 (the only monoclonal antibody currently available for
serodiagnosis of human anisakiasis), based on the calculation of multiples of normal activity for human anisakiasis sera,
indicated that our monoclonal antibodies (and particularly UA3) recognized antigens that are good candidates for serodiagnostic
purposes.
Received: 13 February 1997 / Accepted: 16 March 1997 |
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