COX-2的表达与甲状腺乳头状癌中淋巴管生成及淋巴结转移的关系

目的 探讨环氧化酶-2(COX-2)对甲状腺乳头状癌淋巴管生成的影响及与淋巴结转移的关系.方法 运用Envision免疫组化法测定60例甲状腺乳头状癌患者和15例癌旁正常甲状腺组织病理标本COX-2、血管内皮生长因子C(VEGF-C)的蛋白表达,并进行评分,同时用D2 40显色淋巴管进行淋巴管密度(LVD)计数.结果 甲状腺乳头状癌中COX-2、VEGF-C较正常组织表达明显增高;有淋巴结转移的甲状腺乳头状癌患者中COX-2、VEGF-C的阳性率明显高于无转移组(P<0.05;P<0.05);转移组LVD计数显著高于无转移组(P<0.01);COX-2与LVD(r=0.71;P<0.01)、VEGF-C与LVD(r=0.68;P<0.01)、COX-2与VEGF-C之间(r=0.65,P<0.01)存在显著正相关.结论 COX-2对甲状腺乳头状癌患者的淋巴管形成起着重要的作用,可能通过上调VEGF-C的表达促进肿瘤淋巴管的形成,有利于甲状腺乳头状癌淋巴结的转移.

COX-2 expression in papilary thyroid carcinoma and its relationship with lymphangiogenesis and lymph node metastasis

Objective To study expression of cyclooxygenase-2(COX-2) and its relationship with lymphangiogenesis and lymph node metastasis in papillary thyroid carcinoma.Methods The expressions of COX-2 and VEGF-C were immunohistochemically detected by use of envision method in 60 papillary thyroid carcinoma cases and 14 normal tissue cases nearby tumors respectively,meanwhile the number of lymph vessel density with D2-40 staining positive was obtained.Results The expressions of COX-2 and VEGF-C were higher than that of normal tissues.The positive rates of COX-2 and VEGF-C with lymph node metastasis in papillary thyroid carcinoma were siginificantly higher than that without lymph node metastasis(P<0.05;P<0.05).The number of LVD with D2-40 staining in papillary thyroid carcinoma with lymph node metastasis was remarkablely higher than that without lymph node metastasis(P<0.01).There were siginifcantly positive relationship between COX-2 and LVD in papillary thyroid carcinoma(r=0.71,P<0.01),as well as siginifcantly positive relationship between VEGF-C and LVD(r=0.68,P<0.01).In the meantime the expression of COX-2 was closely related with the expression of VEGF-C in papillary thyroid carcinoma(r=0.65,P<0.01).Conclusion COX-2 may play an important rate in the lymphangiogenesis by up-regulation expression of VEGF-C and promote lymph node metastasis in papillary thyroid carcinoma.