靶向MMP-9脱氧核酶抑制人肺腺癌细胞的粘附与迁移的实验研究

背景与目的脱氧核酶(DNAzyme,Deoxyribozyme)具有高效的催化活性和特异的序列识别能力,可特异性切割靶RNA使其失去生物学活性,从而抑制靶RNA的基因表达。本实验通过探讨靶向MMP-9的脱氧核酶对人肺腺癌A549细胞的细胞粘附与迁移能力的影响,为使用脱氧核酶抑制肺癌的浸润转移提供理论依据。方法设计靶向MMP-9的脱氧核酶,应用oligofectamine将脱氧核酶转染到A549细胞中,采用Western blot方法检测细胞中MMP-9的表达,以及利用细胞粘附与迁移试验观察脱氧核酶干预后细胞粘附与迁移能力的变化。结果在靶向MMP-9脱氧核酶干预后,细胞中MMP-9的表达较对照组与空白组显著减低(P<0.01),同时发现细胞的粘附率、迁移速度均显著降低(P<0.01)。结论靶向MMP-9的脱氧核酶能够抑制人肺腺癌A549细胞中MMP-9的表达,并且有效阻止细胞的粘附与迁移。

Experimental study of targeting MMP-9 deoxyribozyme role of adhesion and migration in human lung adenocarcinoma cancer cell

Background and objective Deoxyribozyme has high biocatalytic activity and sequence specificity against the target mRNA and inhibits gene expression at mRNA level.The aim of this study is to investigate the impact of targeting MMP-9 deoxyribozyme to cell adhesion and migration in human lung adenocarcinoma cell line A549.Methods The targeting MMP-9 deoxyribozyme was designed by oligofectamine into human lung adenocarcinoma cell line A549.The expression of MMP-9 in cell was detected by Western blot.The cell adhesion and migration after the intervention of deoxyribozyme was observed.Results After targeting MMP-9 deoxyribozyme intervention,the expression of MMP-9 in the cells compared with the control group was significantly lower(P<0.01),at the same time,the rate of cell adhesion and migration were significantly decreased.Conclusion Targeting MMP-9 deoxyribozyme inhibited the expression of MMP-9 in human lung adenocarcinoma cell line A549 and effectively prevent cell adhesion and migration.