单纯化脓性胆囊炎手术标本厌氧培养结果32例分析

目的 研究单纯化脓性胆囊炎手术标本病原菌及耐药状况。方法在化脓性胆囊炎患者术中，用注射器于患处部无菌抽取标本，而后做需氧和厌氧培养。对只在厌氧环境生长的专性厌氧菌按参考文献和有关资料生化反应定种。药敏试验采用纸片琼酯糖扩散法，按Sutter 1975年制定的标准判断结果，需氧菌分离按常规进行。结果32份标本中，细菌培养阳性30例，共检出48株细菌，总检出率93．8％，厌氧菌占47、9％，需氧菌占52．1％。厌氧菌药敏试验结果显示，灭滴灵和氯霉素对绝大多数厌氧菌敏感，次为氨苄青霉素。结论胆囊感染患者病原菌的种类较多，厌氧菌对抗菌药物有较稳定的敏感性。

Results of 32 Operative Specimens Cultured Anaerobically from Simple Pyogenic Cholecystitis

Objective To analyse pathogenic bacteria separation and drug-resistance of operative specimens from simple pyogenic cholecystitis.Methods Specimens were withdrawed aseptically with syringe from affected parts of pyogenic cholecystitis then were cultured aerobically and anaerobically.Obligate anaerobes which only grew inanaerobic environment were identified with reference materials and were separated aerobically with routine analysis.Results Positive results were 30 cases and 48 bacteria were detected from 32 specimens.Its total detection rate was 93.8%.Anaerobic bacteria were 47.9% and acrobic ones were 52.1%.The result of anaerobic drug-sensitivity tests indicated that miediling and cholomycetin were sensitivive to majority of anaerobic bacteria, then was Ampicillin.Conclusion Pathogenic bacteria of cholecystitis patients are of many categories and amaerobic bacteria are stably sensitive to antiseptie.