多囊卵巢综合征患者卵泡液MMP-2、9及其抑制物水平的研究

目的测定多囊卵巢综合征(PCOS)患者取卵日卵泡液基质金属蛋白酶-2、9(MMP-2、9)及基质金属蛋白酶抑制因子-1、2(TIMP-1、2)的水平,探讨其在PCOS发病中可能的作用机制。方法收集30例因PCOS接受体外受精-胚胎移植(IVF-ET)治疗患者取卵日卵泡液,以30例同期因输卵管因素接受IVF的卵巢功能正常患者作为对照组,用ELISA方法检测卵泡液中MMP-2、9和其抑制物TIMP-2、1的水平,并对比是否存在差别。结果 PCOS组卵泡液MMP-2、MMP-9水平高于对照组,差异均有统计学意义(P<0.05),而TIMP-2、TIMP-1水平低于对照组(P>0.05);PCOS组卵泡液MMP-2/TIMP-2、MMP-9/TIMP-1值均高于对照组,差异均有统计学意义(P<0.05)。卵泡液MMP-2与TIMP-2水平呈负相关(r=-0.4037,P<0.05),MMP-9与TIMP-1亦呈负相关(r=-0.4279,P<0.05)。结论 PCOS患者卵泡液MMP-2、9水平升高表明,PCOS的多卵泡发育可能与溶解明胶的活性及细胞外基质重构的异常有关。

Study on the levels of matrix metalloproteinase 2,9 and their inhibitors in the follicular fluid of patients with polycystic ovarian syndrome

Objective To investigate the levels of matrix metalloproteinase（MMP）-2,MMP-9 and their tissue inhibitors（TIMP-2 and TIMP-1） in follicular fluid of patients with polycystic ovary syndrome（PCOS） and normally ovulating women who receive in vitro fertilization-embryo transfer（IVF-ET） and to explore the possible action mechanism of MMPs in the pathogenesis of PCOS.Methods The follicular fluids were collected from 30 women who received IVF-ET because of PCOS（PCOS group） and 30 normally ovulating women who received IVF due to fallopian tube factors（control group）.The levels of MMP-2,MMP-9 and TIMP-2,TIMP-1 were detected by ELISA.Results The levels of MMP-2 and MMP-9 in PCOS group were significantly higher than those of control group（P0.05）.The levels of TIMP-2,TIMP-1 in follicular fluid in PCOS group were lower than those in control group,but there were no significant differences between the two groups（P0.05）.The ratios of MMP-2/TIMP-2,MMP-9/TIMP-1 in PCOS group were significantly higher than those of control group,and there were significant differences between two groups（P0.05）.The levels of MMP-2 in follicular fluid were negatively correlated with those of TIMP-2（r=0.4037,P0.05）,so were the levels of MMP-9 with those of TIMP-1（r=0.4279,P0.05）.Conclusion The increase of levels of MMP-2 and MMP-9 in follicular fluid of patients with PCOS suggests that the multi-follicular development of PCOS patients may be related with gelatinolytic activity and the abnormality of extracellular matrix reconstruction.