骆驼刺中总还原糖与芦丁的含量测定

目的：建立测定骆驼刺中总还原糖与芦丁含量的方法,并对不同产地不同月份的样品含量进行比较。方法：采用紫外分光光度法测定骆驼刺中总还原糖的含量,以葡萄糖为对照品,检测波长为620nm;采用高效液相色谱法测定芦丁的含量,色谱柱为SymmetryShieldRp-18（150mm×3.9mm,5μm）,流动相为乙腈-0.1%磷酸水溶液（19∶81,V/V）,检测波长为360nm,柱温为30℃。结果：葡萄糖检测浓度的线性范围为3.85～70.33μg·mL-（1r=0.9959）,平均加样回收率为99.6%,RSD=1.5%（n=6）;芦丁检测浓度的线性范围为4.95～59.40μg·mL-（1r=0.9996）,平均加样回收率为99.3%,RSD=2.08%（n=6）。不同产地不同月份的骆驼刺中芦丁和总还原糖含量有一定差异,其中新疆乌鲁木齐和且末地区在7-8月份采收的骆驼刺中总还原糖和芦丁的含量较高。结论：本试验结果可为骆驼刺的种植和采收的研究奠定基础。

Content Determination of Rutin and Total Polysaccharides in Alhagi sparsifolia

OBJECTIVE： To establish the methods for the content determination of total polysaccharides and rutin in Alhagi sparsifolia, and to compare the content of each sample from the different producing areas in different months. METHODS： With glucose as control, the content of total polysaccharides was measured by UV spectrophotometry at detection wavelength of 620 nm. The content of rutin was determined by HPLC. The determination was performed on Symmetry Shield Rp18（150 mm×3.9 mm,5 μm） column with mobile phase consisted of acetonitrile-0.1% phosphoric acid （19∶81,V/V） with the detection wavelength at 360 nm and the column temperature at 30 ℃. RESULTS： The linear range of glucose was 3.85～70.33 μg·mL-1（r=0.995 9） with an average recovery rate of 99.6%（RSD=1.5%,n=6）. The linear range of rutin was 4.95～59.40 μg·mL-1（r=0.999 6） with an average rate of 99.3%（RSD=2.08%,n=6）. The contents of total polysaccharides and rutin in A. sparsifolia in different months from different areas had certain differences. A. sparsifolia from Urumqi and Qiemo in Jul. and Aug comtained high content of total polysaccharides and rutin. CONCLUSION： The trial lays foundation for the research for cultivation and harvest of A. sparsifolia.