Relationship Between Hypermethylated MGMT Gene and Osteosarcoma Necrosis Rate After Chemotherapy |
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Authors: | Qiu Cui Weihao Jiang Jun Guo Cheng Liu Dingfeng Li Xiaohong Wang Yanjun Zeng |
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Institution: | (1) Department of Orthopedics, the Affiliated Hospital of Academy of Military Medical Sciences, PLA 307th Hospital, Beijing, 100071, China;(2) Biomechanics and Medical Information Institute, Beijing University of Technology, Beijing, 100022, China; |
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Abstract: | To investigate the relativity of MGMT(O-6-methylguanine-DNA methyltransferase) gene methylation from patients with protein
expression and osteosarcoma necrosis rate after chemotherapy. Fifty-one oteosarcoma tissues were collected, Methylation of
MGMT gene promoter was detected by methylation-specific PCR method, and protein expression of MGMT was examined by immunohistochemistry
procedure, the relationship between methylated MGMT gene expression and patients response to chemotherapy was analyzed. The
positive ratio of methylation MGMT gene promoter in 51 patients was 23.5% (12 in 51). Negative percentage of protein expression
of MGMT was 27.5% (14 in 51). It seemed that methylation of MGMT gene in osteosarcoma tissues had no evident relationship
with the patient’s age, sexuality, and the size and type of neoplasms, etc. The necrosis rates of methylated MGMT of osteosarcoma
(tumor grade from I to IV) were 0 (0/51), 3.9% (2/51), 5.9% (3/51), 13.7% (7/51), respectively. In contrast, the necrosis
rates of unmethylated MGMT of osteosarcoma (tumor grade from I to IV) were 45.1% (23/51), 25.5% (13/51), 3.9% (2/51), 2.0%
(1/51), respectively. It suggest that methylated and unmethylated MGMT gene of osteosarcoma have significant difference in
protein expression. The unmethylated MGMT gene has higher positive protein expression (u = −4.92, P < 0.001). Methylation of MGMT gene has higher tumor necrosis rate in osteosarcoma patients. Methylation in MGMT promoter
may be important for judging the effect of chemotherapy in Osteosarcoma patients. |
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