Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis |
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Authors: | Wei-Ping Chen Jia-Peng Bao Jin-Li Tang Peng-Fei Hu Li-Dong Wu |
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Institution: | (1) Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Jie Fang Road 88#, 310009 Hangzhou, People’s Republic of China; |
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Abstract: | The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental
osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after
surgery, rabbits received intra-articular injection with TSA dissolved in the dimethylsulphoxide (DMSO) in the right knees
and DMSO in the left knees once a week for 5 weeks. Rabbits were killed 7 days after the last injection. The knee joints were
assessed by morphological and histological examination. Messenger RNA expression of cathepsins K, B, L, S and cystatin C was
studied by real-time PCR. TSA inhibited the expression of cathepsins K, B, L, S and cystatin C accompanied with the less degradation
in cartilage. The results suggest that TSA exhibits protective effects against cartilage degradation in rabbits with OA and
the effects may be associated with the inhibition of cathepsins. |
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Keywords: | |
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