妊娠17～37周胎儿可溶性白介素2受体和NK细胞含量的变化

目的：探讨妊娠17～37周正常和宫内感染时胎儿外周血可溶性白介素2受体（Soluble interleuldn-2 receptor，sIL-2R）和自然杀伤细胞（Natural killer，NK）含量的变化。方法：超声引导下行脐带穿刺术，收集129例胎儿外周血，包括97例正常对照组胎儿血，32例宫内感染组（单纯疱疹病毒感染、弓形虫感染、风疹病毒感染）胎儿血，采用双色免疫荧光标记流式细胞仪技术测定胎JLPF周血NK细胞百分率，双抗体夹心酶联免疫吸附试验测定胎JLPF周血中sIL-2R的含量，分析生理状态下胎儿外周血NK细胞、sIL-2R的状况和宫内感染时NK细胞和sIL-2R含量的变化。结果：妊娠17—37周胎儿外周血NK细胞、sIL-2R含量不随孕周改变，r（NK）=-0．03，P〉0．05；r（sIL-2R）=0．167，P〉0．05，宫内感染时NK细胞含量减少，sIL-2R含量增多，与正常对照组相比差异有显著意义；t（NK）=4．29，P〈0．01；t（sIL-2R）=-5．833，P〈0．01。结论：妊娠17—37周胎儿外周血有一定量的NK细胞和sIL-2R存在，但机体免疫功能仍不完善，宫内感染时机体容易出现免疫抑制状态。

Changes of soluble interleukin-2 receptor and natural killer cell in fetuses between 17-37 gestational weeks

Objective:To investigate the contents of soluble interleukin-2 receptor(sIL-2R) and natural killer(NK) cell in normal and infected fetuses during 17-37 gestational weeks.Methods:One hundred and twenty nine fetal blood samples were recruited by cordocentesis and among them,ninety seven were normal fetuses which were included in the control group.Thirty two were involved in the infective group which were infected by Herpes simplex virus,Toxoplasma gondii,or Rubella virus.NK cell was determined by two-colour fluorescence flow cytometry.sIL-2R level was determined by enzyme linked immuno-absorbent assay.Results:NK cell and sIL-2R level remained constant during 17 to 37 weeks' gestation:r(NK)=-0.03,(P>0.05);r(sIL-2R)=0.167,P>0.05.Compared with the control group,the infected fetuses were characterized by a smaller percentage of NK cell and a high level of sIL-2R:t(NK)=4.29,P<0.01;t(sIL-2R)=-5.833,P<0.01.Conclusion:During 17 to 37 gestational weeks,there are some of NK cell and sIL-2R in fetal peripheral blood.The cell-mediated immunity of fetuses is not so strong that when in face of infection fetuses are easier to be immunosuppressive.