P~(53)基因突变及P~(53)突变蛋白在卵巢上皮肿瘤中表达检测方法的探讨 |
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引用本文: | 金镇,李守柔,黄冰玉,冷为春,张爱臣. P~(53)基因突变及P~(53)突变蛋白在卵巢上皮肿瘤中表达检测方法的探讨[J]. 癌变.畸变.突变, 1998, 0(4) |
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作者姓名: | 金镇 李守柔 黄冰玉 冷为春 张爱臣 |
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作者单位: | 中国医科大学第二临床学院妇产科,白求恩医科大学第二临床学院妇产科研究室,白求恩医科大学第三临床学院妇产科 |
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摘 要: | 采用改进的aPCR-SSCP银染技术和SP免疫组化技术检测36例卵巢上皮癌,12例卵巢上皮交界瘤和15例良性卵巢上皮肿瘤的P53基因的突变和突变蛋白的表达。结果卵巢上皮癌P53基因突变率为30.6%,突变蛋白表达为58.4%。P53基因突变主要发生在外显子5~8上,以第7外显子突变率最高。交界性肿瘤个别出现P53基因突变和突变蛋白表达,而在良性肿瘤无一例出现。提示P53基因突变是卵巢癌形成过程中的晚期事件。P53基因突变可作为肿瘤标记物判定卵巢癌的预后。aPCR-SSCP非同位素基因突变技术克服了常规PCR-SSCP检测方法的缺陷,可提高准确性。
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关 键 词: | 卵巢上皮肿瘤;基因;P~(53);突变 |
STUDY OF TEXTING METHOD OF EXPRESSION OF P 53 GENE MUTATION AND MUTATION PROTEIN IN EPITHELLIAL OVARIAN TUMOR |
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Abstract: | To dectect P 53 gene mutation and its mutation protein,improved aPCR-SSCP silver staining and SP immunohistochemical methods were emploied in 12 borderline epithelial ovarian tumor and 15 benign epithelial ovarian tumor.The results showed that positive rate of P 53 gene mutation was 30.6%, and that of the mutation protein was 58.4%. The mutation of P 53 gene was mainly on its exon 5-8,especially on exon 7. In some cases of borderline tumor, P 53 gene and its mutation protein were positive, but nine in benign tumor This indicated that P 53 gene mutation was a later event in the form ovarian carcinoma. P 53 gene mutation might serve as a genetic marker to determine the prognosis of ovairan carcinoma.aPCR-SSCP sliver staining method overed the defect of former method,and improved the accuracy of esamine. |
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Keywords: | Epithelial ovarian tumor Gene P 53 Mutation |
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