P~(53)基因突变及P~(53)突变蛋白在卵巢上皮肿瘤中表达检测方法的探讨

采用改进的ａＰＣＲ－ＳＳＣＰ银染技术和ＳＰ免疫组化技术检测３６例卵巢上皮癌，１２例卵巢上皮交界瘤和１５例良性卵巢上皮肿瘤的Ｐ５３基因的突变和突变蛋白的表达。结果卵巢上皮癌Ｐ５３基因突变率为３０．６％，突变蛋白表达为５８．４％。Ｐ５３基因突变主要发生在外显子５～８上，以第７外显子突变率最高。交界性肿瘤个别出现Ｐ５３基因突变和突变蛋白表达，而在良性肿瘤无一例出现。提示Ｐ５３基因突变是卵巢癌形成过程中的晚期事件。Ｐ５３基因突变可作为肿瘤标记物判定卵巢癌的预后。ａＰＣＲ－ＳＳＣＰ非同位素基因突变技术克服了常规ＰＣＲ－ＳＳＣＰ检测方法的缺陷，可提高准确性。

STUDY OF TEXTING METHOD OF EXPRESSION OF P 53 GENE MUTATION AND MUTATION PROTEIN IN EPITHELLIAL OVARIAN TUMOR

To dectect P 53 gene mutation and its mutation protein,improved aPCR-SSCP silver staining and SP immunohistochemical methods were emploied in 12 borderline epithelial ovarian tumor and 15 benign epithelial ovarian tumor.The results showed that positive rate of P 53 gene mutation was 30.6%, and that of the mutation protein was 58.4%. The mutation of P 53 gene was mainly on its exon 5-8,especially on exon 7. In some cases of borderline tumor, P 53 gene and its mutation protein were positive, but nine in benign tumor This indicated that P 53 gene mutation was a later event in the form ovarian carcinoma. P 53 gene mutation might serve as a genetic marker to determine the prognosis of ovairan carcinoma.aPCR-SSCP sliver staining method overed the defect of former method,and improved the accuracy of esamine.