石蒜属种间亲缘关系AFLP分析

目的 研究石蒜属13种（含2变种）植物的种间亲缘关系和同种不同采集地材料的遗传多样性，为种质资源的开发利用提供分子佐证。方法 采用扩增长度多态性（AFLP）标记对石蒜属植物20份材料的基因组DNA进行种间亲缘关系研究。结果 从33对选择性扩增引物组合中筛选出6对，共扩增得到330条清晰可统计的条带，其中多态性条带为320条，占总带数的96.8%；AFLP分子标记检测到石蒜属20份材料种间遗传相似系数为0.38～0.89；AFLP分子标记聚类结果显示同一采集地的材料亲缘关系更近；石蒜属植物存在较丰富的遗传多样性。结论 AFLP分子标记能有效区分石蒜属植物种间的亲缘关系。

Analysis of genetic relationships among species of Lycoris Herb. by AFLP markers

Objective To investigate the interspecific relationships among thirteen species (include two varieties) of Lycoris Herb. from different habitats and provide the evidence for evaluation and exploitation of the germplasm resources for Lycoris Herb. Methods The genetic diversities of 20 accessions of Lycoris Herb. from different habitats were investigated with the technique of amplified fragment length polymorphism (AFLP). Results Six pairs of AFLP primers were selected from 33 pairs of combinations, and a total of 330 amplified clear DNA bands were obtained, among them 320 were polymorphic bands. The percentage of polymorphic bands was 96.8%. The coefficient ranges of interspecific genetic similarity (GS) in AFLP analyses for the 20 materials were 0.38-0.89. AFLP clustering results showed that the materials from the same habitats had a closer genetic relationship. There were abundant genetic diversity in species of Lycoris Herb. Conclusion AFLP technique is efficient in generating more accurate information on the genetic diversity and relationship in plants of Lycoris Herb.