氨溴索对大鼠胃内容物吸入性肺损伤细胞凋亡的影响

目的研究细胞凋亡在胃内容物吸入性肺损伤中的作用机制,并初步探讨氨溴索对肺损伤发生后细胞凋亡的影响。方法 30只健康Sprague-Dawley大鼠随机分为3组:对照组、损伤组、氨溴索组。采用蛋白免疫印迹法(Western blot)检测Bc12(B cell lymphoma/lewkmia-2)蛋白、Bax(Bc12 associated x protein)蛋白及Caspase-3蛋白表达;DNA原位末端标记(TUNEL)法测定肺组织细胞凋亡指数。结果与对照组比较,损伤组中Bax蛋白、Caspase-3蛋白表达增多,细胞凋亡指数增高;Bc12蛋白表达减少;差异均显著(P<0.05)。与损伤组比较,氨溴索组Bax蛋白、Caspase-3蛋白表达减少,细胞凋亡指数降低;Bc12蛋白表达增高;差异均显著(P<0.05)。结论细胞凋亡参与了胃内容物吸入后肺损伤发病过程,氨溴索在吸入性肺损伤组织中发挥抗细胞凋亡作用,可能机制在于调控死亡信号通路中Bax/Bc12蛋白表达相关。

Influence of ambroxol on cell apoptosis in rats with gastric aspiration lung injury

Objective To study the mechanism of action of cell apoptosis in rats with gastric aspiration lung injury and to investi- gate the influence of ambroxol on cell apoptosis after lung injury. Methods 30 healthy Sprague-Dawley rats were randomly divided and e- venly into the control group, the injury group and the ambroxol group. The expressions of B cell lymphoma/lewkmia-2 （Bcl-2） , Bcl2 as- sociated x protein （Bax） and Caspase-3 were determined by Western blot, and the indexes of cell apoptosis in lung tissus were detected by TUNEL. Results The expressions of Bax and Caspase-3 were significantly higher, and the expression of Bcl-2 was obviously lower in the injury group than in the control group （ P 〈 0.05 ）. Compared with the injury group, the expressions of Bax and Caspase-3 and cell apop- totic index decreased notably in the ambroxol group （P 〈 0. 05 ） , but the expression of Bcl-2 increased obviously （P 〈 0. 05 ）. Conclusion The cell apoptosis plays an important role in the course of gastric aspiration lung injury, and ambroxol has anti-apoptotic effect on lung injury. The probable mechanism is to regulate the expression of Bax/Bcl-2 of death signaling pathway.