生物新材料细菌纤维素与深Ⅱ度烧伤大鼠皮肤的创面愈合

背景:研究表明细菌纤维素有利于皮肤组织生长和限制感染,为制备用于烧伤患者和慢性皮肤溃烂患者的生物敷料及临时皮肤提供有利条件.目的:通过动物实验观察细菌纤维素作为烧伤敷料的可能性.设计、时间及地点:观察实验,于2008-06/12在上海交通大学附属第六人民医院完成.材料:菌株M12经30℃恒温静置培养6 d发酵可产生不溶性的凝胶膜,用0.1 mol/L的NaOH溶液,100℃煮沸20 min,去除液膜中的菌体和残留培养基后,即得乳白色半透明的细菌纤维素膜.方法:用自制烫伤仪于30只SD大鼠背部脊柱两侧各制造2.0 cm×2.0 cm大小的皮肤烫伤,随机选择一侧为治疗侧,表面敷以细菌纤维素膜,用1号手术缝线缝扎固定于周围皮肤上,另一侧为对照侧,不予包扎.主要观察指标:术后4,7,14,21,28 d计算伤口愈合率.光镜下观察皮肤损害及愈合情况.结果:所有实验动物无死亡,均未出现创面感染,术后21 d治疗侧伤口愈合率高于对照侧,差异有显著性意义(P<0.01);其他时间点两侧比较差异无显著性意义.组织学观察两侧病理损害和愈合程度在各个时间点无明显差异.结论:细菌纤维素膜在一定程度上具有促进烧伤创面愈合的作用.

Effect of bacterial cellulose on the wound healing of deep second-degree burn in rats

BACKGROUND: Bacterial cellulose, a new nanometer biomaterial, benefits the growth of skin tissue and limits the infection. It may provide advantageous conditions for the biomaterials dressing and temporary skin in the burnt patients and chronic ulcer patients. OBJECTIVE: To study the possibility of bacterial cellulose in the treatment of deep second-degree burn in rats. DESIGN, TIME AND SETTING: An observational experiment was perforemd at the Central Laboratory of Sixth People's Hospital of Shanghai Jiao Tong University from June to November 2008. MATERIALS: M12 was used to produce non-soluble gel membrane when it was cultured for 6 days at a constant temperature of 30℃, and then the sample was boiled for 20 minutes in 0.1 mol/L NaOH solution. Ivory semitransparent bacterial cellulose was obtained following the removals of thallus in liquid membrane and residual culture medium. METHODS: Skin burn in a size of 2.0 cm×2.0 cm was made in bilateral sides of spinal cord on back with self-made wound meter. One side was treated with bacterial cellulose dressing and sutured, taking as experimental side. While the other side served as control side without dressing. MAIN OUTCOME MEASURES: At days 4, 7, 14, 21 and 28 after operation, the healing rate of skin burn in rats was calculated, and the skin wound and healing were observed under light microscope. RESULTS: All animals were alive normally without the presence of wound infection. Compared with control side, the wound healing rate of treated side significantly improved at day 21 after operation; there was no significant difference between the treated side and control side at other time points (P < 0.01). The histological results indicated that the pathological lesion and healing had no significant differences between the two sides. CONCLUSION: Bacterial cellulose can promote the healing of skin burn wound to a certain degree.