| HPLC法研究苯乙烯-DNA的加合特性 |
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| 引用本文： | 张放,邵华.HPLC法研究苯乙烯-DNA的加合特性[J].中国卫生检验杂志,2007,17(3):385-387. |
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| 作者姓名： | 张放 邵华 |
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| 作者单位： | 山东省职业卫生与职业病防治研究院,济南,250062 |
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| 基金项目： | 国家自然科学基金;山东省科技攻关项目 |
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| 摘 要： | 目的：研究苯乙烯的DNA加合特性。方法：采用高效液相色谱法研究苯乙烯-7，8-氧化物（S0）、苯乙烯、苯乙醇酸（MA）、苯乙醛酸（PGA）和DNA的加合反应。结果：苯乙烯、MA、PGA不与DNA发生加合反应；SO可与脱氧鸟苷酸及脱氧腺苷酸发生加合反应，生成以共价键结合的加合物。结论：苯乙烯进入机体后，通过其活性中间代谢物SO与DNA起加合作用，SO攻击DNA脱氧鸟苷酸及脱氧腺苷酸形成加合物，如果在细胞复制前所形成的DNA加合物没有被修复或者被错误修复的话，就有可能导致基因突变。苯乙烯的其他代谢物未见此效应。
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| 关 键 词： | 苯乙烯 DNA加合物 |
| 文章编号： | 1004-8685（2007）03-0385-03 |
| 收稿时间： | 2006-10-30 |
| 修稿时间： | 2006年10月30 |
A study on adduct characteristics between styrene and DNA by HPLC |
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| Zhang Fang,Shao Hua.A study on adduct characteristics between styrene and DNA by HPLC[J].Chinses Journal of Health Laboratory Technology,2007,17(3):385-387. |
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| Authors: | Zhang Fang Shao Hua |
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| Institution: | Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China |
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| Abstract: | Objective:To study the adduct characteristics between styrene and DNA.Methods:The adduct reactions between styrene,urinary mandalic acid(MA),phenylglyoxalic acid(PGA) and DNA by were studied HPLC analysis.Results:SO could unite dGMP and dAMP with covalent linkage,but styrene,urinary mandalic acid(MA) and phenylglyoxalic acid(PGA) could not.Conclusion:SO,as the active intermediate metabolite of styrene in human body,can unites with dGMP and dAMP to form adducts.If these DNA adducts are not repaired or are mis-repaired before cell getting duplication,the gene mutation would happen.There are not the same processes between other metabolites of styrene and DNA. |
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| Keywords: | HPLC |
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