眼眶及眼附属器黏膜相关B细胞淋巴瘤中BCL10表达和染色体易位的检测

目的 研究眼眶及眼附属器黏膜相关(OA-MALT)淋巴瘤中BCL10蛋白表达及染色体易位情况.方法 收集60例OA-MALT淋巴瘤患者的标本,采用免疫组织化学法检测BCL10蛋白的表达,用荧光素原位杂交(FISH)法分别检测AP12-MALT1融合基因、BCL10、MALT1及IgH基因的异常.采用交叉分组设计,对结果进行Fisher精确检验.结果 OA-MALT淋巴瘤中BCLI0蛋白表达的阳性率为85.0%(51/60),其中胞质阳性率为41.7%(25/60),胞核阳性率为43.3%(26/60).FISH结果显示除2例患者有API2.MALTI融合基因外,其余患者的BCL10基因和IgH基因均未发现异常.统计学分析显示OA-MALT淋巴瘤中BCL10蛋白核表达不依赖于染色体异常(P=0.184).结论 OA.MALT淋巴瘤中有较高的BCL10蛋白核表达率,对OA-MALT淋巴瘤的诊断有一定的辅助价值.在全身其他部位MALT淋巴瘤中报道的染色体易位,在OA-MALT淋巴瘤中并不常见.

Abnormal BCL10 nuclear expression and chromosomal translocation in ocular adnexal mucosa-associated lymphoid tissue lymphomas

Objective To detect the BCL10 expression and several types of chromosomal translocations including t (11 ;18)/API2-MALT1 ; t (1 ;14)/IgH- BCL10 and t (14;18)/MALTI-IgH] in ocular adnexal mucosa-associated lymphoid tissue lymphomas (OA-MALT lymphomas). Methods Sixty OA-MALT lymphomas were collected from Cancer Hospital of Fudan University. BCLIO was detected by immunohistochemical studies, and API2-MALT1 fusion gene, BCLIO, MALT1 and IgH chromosomal abnormalities were detected by fluorescent in situ hybridization ( FISH). Fisher's exact test was used to analyze the relation between nuclear BCL10 expression and chromosomal translocation. Results BCL10 expressed in 85.0% (51/60) OA-MALT lymphomas. Among these positive cases, 25 cases (41.7%) were expressed in the cytoplasm, and 26 cases (43.3%) were expressed in the nucleus. FISH results showed that no chromosomal abnormalities related with BCL10 and IgH genes, except 2 cases with API2-MALT1 fusion gene. Under statistic of Fisher exact test, nuclear BCL10 expression and API2-MALT1 fusion gene were two independent factors ( P > 0.05 ). Conclusions BCL10 nuclear expression is common in OAMALT lymphomas and may be used as a potential marker for the diagnosis of MALT lymphomas arising from ocular adnexa. Aberrant chromosomal translocations reported in the other sites MALT lymphomas are rare in OA-MALT lymphomas.