Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system |
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| Authors: | Anne J. Blaschke Caroline Heyrend Carrie L. Byington Mark A. Fisher Elizabeth Barker Nicholas F. Garrone Stephanie A. Thatcher Andrew T. Pavia Trenda Barney Garrison D. Alger Judy A. Daly Kirk M. Ririe Irene Ota Mark A. Poritz |
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| Affiliation: | 1. Department of Pediatrics, University of Utah, Salt Lake City, UT, USA;2. Idaho Technology, Inc., Salt Lake City, UT, USA;3. Department of Pathology, University of Utah, Salt Lake City, UT, USA;4. Associated Regional and University Pathologists, Salt Lake City, UT, USA;5. Primary Children''s Medical Center Microbiology Laboratory, Salt Lake City, UT, USA |
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| Abstract: | Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture. |
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| Keywords: | Polymerase chain reaction Sepsis Bacteremia Blood culture Molecular diagnostics Bloodstream infection FilmArray |
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