| 组织原位测算单个肝细胞核的DNA总量 |
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| 引用本文： | 赵品,夏潮涌.组织原位测算单个肝细胞核的DNA总量[J].中国体视学与图像分析,2010(1):74-78. |
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| 作者姓名： | 赵品 夏潮涌 |
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| 作者单位： | 暨南大学医学院组织学与胚胎学教研室; |
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| 摘 要： | 目的再次探讨利用图像分析系统在组织切片原位测算以单个完整细胞（核）体积为单位的化学物质总量方法的可靠性。方法选取10只成年健康雄性昆明小鼠,每只小鼠按常规制片方法制作4μm和11μm两种厚度的肝组织切片各一张,改良Feulgen染色,应用细胞图像分析仪在组织原位测量和计算以单个完整二倍体、四倍体和八倍体肝细胞核体积为单位的DNA总量。结果组织原位测算的以单个完整二倍体、四倍体、八倍体肝细胞核体积为单位的DNA总量间的比值基本上呈2或4的倍数关系。结论应用细胞图像分析仪在组织切片原位通过合适的抽样和测量计算,可较准确地获得以单个完整细胞（核）体积为单位的化学物质总量。
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| 关 键 词： | 组织原位 DNA含量 图像分析 |
Measurement of DNA content in volume unit of whole hepatocyte nuclei on the liver tissue in situ |
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| ZHAO Pin,XIA Chaoyong.Measurement of DNA content in volume unit of whole hepatocyte nuclei on the liver tissue in situ[J].Chinese Journal of Stereology and Image Analysis,2010(1):74-78. |
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| Authors: | ZHAO Pin XIA Chaoyong |
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| Institution: | ZHAO Pin,XIA Chaoyong(Department of Histology , Embryology,Medical College of Jinan University,Guangzhou 510362,China) |
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| Abstract: | Objective Objective Re-estimation of the reliability of measuring the DNA content in volume unit of whole hepatocyte nuclei on the liver tissue in situ by image analysis.Methods The 4 μm and 11 μm liver tissue slices were prepared,respectively,from 10 healthy adult male Kunming mice,and then together stained with modified Feulgen stain.The DNA content in volume unit of whole diploid or tetraploid or octoploid nuclei were measured and calculated by TIGER-cell image analysis system,respectively.Results The ratio of DNA content in volume unit of whole diploid,tetraploid and octoploid nuclei were close to 2 or 4.Conclusions With using an image analysis system,we can obtain the content of chemical substances in volume unit of whole cells or nuclei on the tissue in situ by suitable sampling and calculation. |
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| Keywords: | tissue in situ DNA contents image analysis |
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