独一味对内毒素血症BALB／c小鼠血清细胞因子的影响

目的检测独一味预处理对内毒素血症BALB／C小鼠血清细胞因子水平的影响，初步探讨独一味对炎症的调节机制。方法用独一味浸膏液对雄性BALB／c小鼠进行灌胃预处理，5d后给予内毒素促使内毒素血症的发生，6h后动物断头取血，制备血清样品，利用LiquiChip系统检测血清细胞因子水平。结果细胞因子检测发现，非致死性内毒素血症6h时血清细胞因子普遍增高，为正常水平的数倍到数百倍（P〈0．01）。给予独一味处理可以改变内毒素血症细胞因子的血清水平，使血清MCP-1约下降20％（P〈0．01），血清TNFa约下降40％（P〈0．01），使血清IL-1β约上升1倍（P〈0．01），血清IL-4上升了37％（P〈0．01），血清IL-10约上升20％（P〈0．01），独一味对血清IL-6水平没有明显影响（P〉0．05）。结论独一味对内毒素血症不同细胞因子的调节不同，具有选择性和多样性。其提高了抗炎细胞因子的水平，减少了部分促炎因子的表达，避免发生过激的炎症反应，保护机体组织，且同时还提高IL-1等促炎细胞因子的表达，增强机体的天然免疫机制。

The effects of Duyiwei to the serum cytokine of BALB/c mice suffered with endotoximia

Objective To explore the mechanism of the modification function on inflammation of Duyiwei by detecting the serum cytokine level of BALB/c mice suffered with endotoximia. Methods The male BALB/c mice were pretreated with extracts of herb Duyiwei (Lamiophlomis rotata kudo) with intragastric administration for 5days, and then were administrated with lipopolysaccharide (LPS) intraperitoneally for 6 hours. The mice were sacrificed by decollation and the bloods were collected to prepare serum samples, which were...