人白细胞抗原G 14bp插入/缺失多态性和血浆可溶性人白细胞抗原G表达水平与系统性红斑狼疮的相关性研究

目的检测系统性红斑狼疮（SLE）患者人白细胞抗原G（HLA-G）14bp插入/缺失（ins/del）多态性和血浆可溶性HLA-G（sHLA-G）水平,并分析它们与SLE的关联性。方法聚合酶链式反应（PCR）检测231例SLE患者及367名健康体检者的HLA-G 14bp ins/del多态性的基因型分布,酶联免疫吸附法（ELISA）检测其中96例SLE患者及74名健康体检者血浆sHLA-G水平。结果HLA-G14bp ins/del多态性位点和基因型频率分布在正常人组和SLE组均无统计学意义（P均〉0.05）,血浆sHLA-G水平在SLE患者组为（230.2±192.2）U/ml,明显高于正常人组的（118.3±38.1）U/ml（P=0.0001）;血浆sHLA-G水平增高的SLE患者易出现中枢神经系统受累（P=0.007）,且较血浆sHLA-G水平正常的患者病情更重（P=0.027）。进一步分析血浆sHLA-G水平增高和正常SLE患者的HLA-G 14bp ins/del多态性位点和基因型分布,未发现两组间有差异（P=0.675）。结论血浆sHLA-G水平在SLE患者中明显增高提示sHLA-G可能参与到SLE发病机制中,其水平高低主要取决于SLE病情严重性及活动性,而与HLA-G1 4bp ins/del多态性无关。

HLA-G 14 bp ins /del polymorphism and expression of plasma soluble HLA-G in patients with systemic lupus erythematosus

Objective To determine the association of HLA-G 14 bp insertion/deletion polymorphism and plasma soluble HLA-G（sHLA-G）levels with systemic lupus erythematosus（SLE）.Methods Polymerase chain reaction（PCR）was performed to detect the genotypes of HLA-G 14 bp ins/del polymorphism in 231 SLE patients and 367 healthy controls,and soluble HLA-G（sHLA-G）levels were determined by enzyme-linked immunosorbent assay in 96 SLE patients and 74 healthy controls.Results No significant differences were found in HLA-G 14 bp ins/del allelic frequencies and genotype distributions between SLE patients group and the control group（P〉0.05）.However,plasma concentration of sHLA-G was significant higher in SLE patients than that in healthy controls（230.2±192.2）U/ml vs.（118.3±38.1）U/ml,P=0.0001）.Moreover,the patients with increased levels of sHLA-G had higher incidence of central nervous system involvement（P=0.007）and more severe disease activity（P=0.027）in comparison with patients with normal plasma sHLA-G levels.Finally,analysis of HLA-G 14 bp ins/del polymorphism in patients with increased sHLA-G levels revealed that there was not different from that in patients with normal sHLA-G levels（P=0.675）.Conclusions The increased production of sHLA-G indicates that sHLA-G may play an important role in the pathogenesis of SLE,the expression of sHLA-G may be associated with disease activity and severity of lupus patients,but be independence of HLA-G 14 bp ins/del polymorphism.