大剂量芹菜素对大鼠抗氧化酶活性及DNA损伤影响的研究

目的观察大剂量芹菜素对大鼠的抗氧化活性及对DNA氧化损伤的影响。方法将100只Wistar大鼠随机分为5组,每组20只,即基础饲料对照组,低剂量芹菜素组(掺入量为2.5%,相当于2g/kgbw),次低剂量芹菜素组(掺入量为5.0%,相当于4g/kgbw),中剂量芹菜素组(掺入量为7.5%,相当于6g/kgbw),高剂量芹菜素组(掺入量为10%,相当于8g/kgbw),实验周期为13周。实验结束前收集动物2h尿液,实验结束后处死动物,留取血液和肝脏,测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽硫转移酶(GST)活性、丙二醛(MDA)含量,分析8-羟基脱氧鸟苷含量。结果在2和4g/kgbw剂量时,雄性肝脏SOD、GSH-Px以及2g/kgbw剂量组的GST活性明显低于对照组(P<0.05);血清中雄性8g/kgbw剂量组的GST以及雌雄4g/kgbw剂量组的GSH-Px活性明显低于对照组(P<0.05),其他各项指标与对照组比较,差异均无显著性。结论本试验条件下,大剂量芹菜素能够降低雄性大鼠的抗氧化酶活性,但对DNA损伤没有影响。

Effect of high dose apigenin on antioxidase activity and DNA damage in rats

Objective To investigate the effect of high doses apigenin on antioxidase activity of and DNA oxidative damages in rats. Methods 100 Wistar rats were randomly divided into five groups including control ,dose one to four groups. The trial lasted 13 weeks,the blood samples and liver of rats were collected at the end of the experiment. The activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),glutathione-S-transferase(GST)and levels of malondialdehyde (MDA) were determinde,8-OHdG content ,which is indicative of DNA damage were assessed. Results At 2g/kg and 4g/kg apigenin,the activity of SOD and GSH-Px in male rat liver significantly decreased,at 2g/kg bw dose,the activity of male rat liver GST also greatly decreased. At the dose of 4g/kg apigenin,GSH-Px activity in serum of both sex rats significantly decreased,its male rat GST activity at dose of 8g/kg apigenin also greatly decreased.Conclusion High dose apigenin could decrease antioxidase activity of male rat. but ,it didn't cause DNA damage.