鼻咽癌患者唾液和血清及外周血白细胞中EBV-DNA的定量检测

目的对鼻咽癌患者唾液、血清及外周血白细胞中的EBV-DNA进行检测,分析唾液、血清、外周血白细胞中EB病毒的DNA含量与鼻咽癌的相关性。方法对40例初诊鼻咽癌患者和50例正常人的唾液、血清及外周血白细胞中的EBV-DNA进行荧光聚合酶链反应(FQ-PCR)检测。结果EBV-DNA在鼻咽癌患者血清中的检出率为67.5%,显著高于正常人血清中的EBV-DNA检出率2%(χ2=44.48,P<0.001);鼻咽癌患者的唾液、外周血白细胞EBV-DNA检出率与正常人相比,无显著性差异;鼻咽癌患者和正常人的唾液EBV-DNA拷贝数与外周血白细胞EBV-DNA拷贝数呈正相关性;鼻咽癌患者血清EBV-DNA拷贝数与TNM分级有关,分级越晚含量越高。结论 EB病毒长期存在于多数人的唾液中,也可存在于外周血B淋巴细胞中。血清EBV-DNA检测在鼻咽癌的临床诊断中具有较高的敏感性和特异性,有助于鼻咽癌的早期诊断;血清EBV-DNA拷贝数与TNM分级有关,可在分子水平对鼻咽癌的TNM分级进行补充。

Quantitative analysis of Epstein-Barr virus DNA in saliva,blood serum and peripheral blood cells in patients with nasopharyngeal carcinoma

Objective To quantitatively analyze DNA levels of Epstein-Barr virus(EBV) in saliva,blood serum,and peripheral blood cells(PBCs) of nasopharyngeal carcinoma(NPC) patients,and to investigate the relationship between EBV-DNA levels and NPC.Methods EBV-DNA levels in samples of saliva,blood serum and PBCs from 40 primary NPC patients and 50 controls were quantitatively detected with real-time quantitative PCR(FQ-PCR) respectively.Results The detective rate of EBV-DNA in blood serum from NPCs(67.5%) was significantly higher than that in the controls 2%(χ2=44.48,P<0.001).The differences of EBV-DNA detective rates in the saliva,PBCs between the NPCs and control group were statistically insignificant.Saliva EBV-DNA level was correlated with PBCs EBV-DNA load in both NPCs and the control.In NPC patients,blood serum EBV-DNA level was significantly increasing with the advance in TNM stage.Conclusion Epstein-Barr virus may exist in saliva and peripheral blood B lymphocyte in most normal people.With high sensitivity and specificity,the blood serum EBV-DNA examination may be helpful in the early diagnosis of NPC,and also facilitate the TNM staging of NPC in clinical practice.