Microarray based IgE detection in poly-sensitized allergic patients with suspected food allergy - an approach in four clinical cases |
| |
| Authors: | Vieira T Lopes C Pereira A M Araújo L Moreira A Delgado L |
| |
| Institution: | 1. Serviço de Imunoalergologia, Hospital São João, E.P.E., Porto, Portugal;2. Serviço de Patologia Clínica (Laboratório de Imunologia), Hospital São João, E.P.E., Porto, Portugal;3. Serviço e Laboratório de Imunologia, Universidade do Porto, Portugal;4. CINTESIS (Centre for Research in Health Technologies and Information Systems), Faculdade de Medicina, Universidade do Porto, Portugal;1. Centro de Histocompatibilidade do Centro, Praceta Prof. Mota Pinto, Edifício São Jerónimo, Piso 4, 3001-301 Coimbra, Portugal;2. Immunoallergology Department, Centro Hospitalar de S. João EPE, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;3. Immunoallergy Department, Hospital D. Estefânia, Centro Hospitalar de Lisboa Central, Rua Jacinta Marto, 1169-045 Lisboa, Portugal;4. Immunoallergology Department, Hospital Santa Maria, Centro Hospitalar Lisboa Norte, Faculty of Medicine of Lisbon, Avenida Prof. Egas Moniz, 1649-035 Lisboa, Portugal;5. Immunoallergology Department, Hospitais da Universidade de Coimbra, Praceta Prof. Mota Pinto, 3000-075, Coimbra, Portugal;1. General and Digestive Surgery Service, Virgen de las Nieves University Hospital, Granada, Spain;2. Experimental Surgery Research Unit, Virgen de las Nieves University Hospital, Granada, Spain;1. Fundació irsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain;2. Fundació Lluita contra la SIDA, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain;1. Barcelona Centre for International Health Research (CRESIB), Hospital Clínic, Universitat de Barcelona, Barcelona, Spain;2. Department of Clinical Microbiology, School of Medicine, IDIBAPS, Barcelona, Spain |
| |
| Abstract: | BackgroundComponent-resolved diagnosis and microarray technology have been recently introduced into clinical allergy practice, and may be particularly useful in poly-sensitized allergic patients.MethodsWe compare the clinical usefulness of a microarray-based IgE detection assay (ISAC®) with skin tests and specific IgE with standard allergens (sIgE) or their monocomponents in four case reports of patients poly-sensitized to aeroallergens and food.ResultsCase 1: a woman with rhinitis, oral allergy syndrome to several fruits and anaphylaxis to cherry. Diagnostic tests supported non-specific lipid transfer proteins (nsLTPs) primary sensitization.Case 2: a woman with exercise-induced asthma, rhino-conjunctivitis and oral allergy syndrome to fresh fruits of different families. A diagnosis of primary grass and weed pollen allergy with profilin and pathogenesis-related protein family 10 (PR-10) cross-reactive food allergy was proposed.Case 3: a man with atopic eczema, asthma, rhinitis, and multiple anaphylactic episodes with cashew nuts and oral allergy syndrome to fruits. The diagnostic workup supported a primary birch pollen allergy with PR-10 and nsLTPs cross-reactive food allergy.Case 4: a woman with rhino-conjunctivitis, per-operative anaphylaxis due to latex and recent pharyngeal angio-oedema episodes. The diagnosis was a primary grass and weed pollen allergy with equivocal profilin sensitization and no obvious cross-reactivity mediated by nsLTPs sensitization.ConclusionsThe possibility to carry out multiple sIgE measurements with single protein allergens, in particular with the microarray technique, is a useful, simple and non-invasive diagnostic tool in complex poly-sensitized allergic patients. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
