Increased genetic diversity of the Mycobacterium tuberculosis W-Beijing genotype that predominates in eastern China |
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| Affiliation: | 1. School of Public Health & Key Laboratory of Public Health Safety (Ministry of Education), Fudan University, Shanghai, China;2. School of Public Health and Primary Care, Chinese University of Hong Kong, China;3. Hongkou District Center for Disease Control and Prevention, Shanghai, China;1. Division of Epidemiology, Dalla Lana School of Public Health, University of Toronto, Toronto, Canada;2. Jockey Club School of Public Health & Primary Care, The Chinese University of Hong Kong, Hong Kong SAR, China;3. Dalla Lana School of Public Health, University of Toronto, Canada;4. School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region;5. Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang Province, China;6. Ningbo Center for Disease Control and Prevention, Ningbo, China;7. Division of Clinical Public Health, and Institute of Health Policy, Management and Evaluation, Dalla Lana School of Public Health, University of Toronto, Toronto, Canada;1. School of Pharmacy, University of Waterloo, 10Victoria St S A, Kitchener, ON, N2G 1C5, Canada;2. Ontario Pharmacy Evidence Network (OPEN), School of Pharmacy, University of Waterloo, 10 Victoria St S A, Kitchener, ON, N2G 1C5, Canada;3. Leslie Dan Faculty of Pharmacy, University of Toronto, 144College St, Toronto, ON, M5S 3M2, Canada;4. Shoppers Drug Mart, Store Number 500, 1630 Danforth Ave, Toronto, ON, M4C 1H6, Canada |
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| Abstract: | ObjectivesTo determine the genetic diversity of Mycobacterium tuberculosis (M. tuberculosis) strains in a Chinese population predominately infected with strains of the W-Beijing family.MethodsA cross-sectional study was conducted in three counties of eastern China. M. tuberculosis strains were collected at TB clinics, and patients were interviewed by trained physicians at the time of TB diagnosis. RD105 and RD181 were used to identify W-Beijing and modern W-Beijing strains, respectively, while seven-locus variable numbers of tandem repeat–mycobacterial interspersed repetitive unit (VNTR–MIRU) analysis was employed to differentiate the genotypes of these strains.ResultsOf 441 strains studied, 394 (89.3%) were identified as W-Beijing family strains; of them, 299 were modern W-Beijing strains. VNTR–MIRU identified 409 genotypes from 426 strains, including 395 unique patterns and 14 clusters. Ancestral W-Beijing strains were more likely to be clustered (OR = 1.32, 95%CI: 0.58–2.97) compared to modern W-Beijing strains. The proportions of clustered strains were 14.6%, 4.2% and 0% at sites Funing (FN), Deqing (DQ) and Yinzhou (YZ), respectively. Of the seven MIRU loci, VNTR3820 was found to have the highest discriminatory power and allelic diversity.ConclusionsVNTR–MIRU typing appears to be a reliable method for analyzing M. tuberculosis transmission in relatively closed populations. The low clustering proportions indicate that endogenous relapse may be a main source of TB cases in eastern China. Furthermore, our results indicate that migration has played may play an important role in the recent transmission of the W-Beijing family of M. tuberculosis. |
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| Keywords: | W-Beijing family VNTR–MIRU Genetic diversity Migrants |
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