Blastocystis subtypes detected in humans and animals from Colombia |
| |
| Institution: | 1. Department of Biological Science, Faculty of Sciences, Nara Women''s University, Kitauoya-Nishimachi, Nara 630-8506, Japan;2. Department of Biological Sciences, Graduate School of Humanities and Sciences, Nara Women''s University, Kitauoya-Nishimachi, Nara 630-8506, Japan;3. Osaka Municipal Tennoji Zoological Gardens, Tennoji-ku, Osaka 543-0063, Japan;1. Department of Biological Science, Faculty of Sciences, Nara Women''s University, Kitauoya-Nishimachi, Nara 630-8506, Japan;2. Department of Parasitology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8640, Japan;3. Eijkman Institute of Molecular Biology, Jakarta, Indonesia;4. Department of Parasitology, Faculty of Medicine, Hasanuddin University, Jalan Perintis Kemerdekaan Km 10, Makassar 90245, Indonesia;1. Statens Serum Institut, Copenhagen, Denmark;2. London School of Hygiene and Tropical Medicine, London, UK;1. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo 83000, Sonora, Mexico;2. Hospital General “Dr. Manuel Gea González”, Calzada de Tlalpan 4800, Ciudad de México 14080, Mexico;3. Servicio de Infectología, Hospital Infantil del Estado de Sonora, Hermosillo 83100, Sonora, Mexico;4. Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A. C., Hermosillo 83304, Sonora, Mexico;5. Hospital Infantil de Morelia “Eva Samano de López Mateos”, Bosque Cuauhtémoc s/n, Morelia 58000, Michoacán, Mexico;6. Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;1. Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Université Clermont Auvergne, CNRS, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand F-63000, France;6. CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Parasitologie-Mycologie, Hôpital G. Montpied, Clermont-Ferrand F-63003, France |
| |
| Abstract: | Blastocystis is a common enteric protist colonizing probably more than 1 billion people along with a large variety of non-human hosts. This protist has been linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, flatulence and irritable bowel syndrome (IBS). Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs in different Colombian hosts. We obtained fecal samples positive for Blastocystis by microscopy from 277 humans, 52 birds, and 117 mammals (25 cattle, 40 opossums, 40 dogs, 10 rats and 2 howler monkeys). The samples were submitted to DNA extraction, PCR and sequencing using primers targeting the small subunit rRNA gene, and ST identification was performed according to DNA barcoding. We observed the occurrence of ST1 (34%) and ST2 (23%) and lower proportions of STs 3 (11.4%), 4 (0.8%), 6 (19.8%) and 8 (10.5%). Domesticated mammals shared the same STs as those usually seen in humans (ST1, ST2, ST3), while birds and marsupials had STs, which are usually rare in humans (ST6, ST8). Further studies implementing high-resolution molecular markers are necessary to understand the phylodynamics of Blastocystis transmission and the role of this stramenopile in health and disease in Colombian populations, and to expand on the phylogeographic differences observed so far with a view to exploring and understanding host–parasite co-evolution. |
| |
| Keywords: | Protist Parasite Ribosomal lineages Subtypes Molecular epidemiology |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
