Correlation of Giardia duodenalis assemblages with clinical and epidemiological data in Cuban children |
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| Affiliation: | 1. Infectious Disease Division, Respiratory and Enteric Infections Department, International Center for Diarrheal Disease Research, Dhaka, Bangladesh;2. Online Research Club (http://www.onlineresearchclub.org/), Nagasaki, Japan;3. Faculty of Medicine, Al-Azhar University, Cairo, Egypt;4. Saigon General Hospital, Ho Chi Minh City, Vietnam;5. University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh, Vietnam;6. Faculty of Health Sciences, McMaster University, Hamilton, ON. Canada;7. School of Medicine, Faculty of Health and Medical Sciences, Taylor''s University, Selangor, Malaysia;8. Department of Biomedical Engineering, South Dakota School of Mines and Technology, Rapid City, 57701, United States;9. Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, 57701, United States;10. Faculty of Medicine, Ain Shams University, Cairo, Egypt;11. Institute of Medical Technology, Baghdad, Iraq;12. Faculty of Medicine, Menoufia University, Yassin Abdel-ghaffar Street, Shebin El-Kom, Menoufia, 32511, Egypt;13. Faculty of Medicine, Ain Shams University, Cairo, Egypt;14. Evidence Based Medicine Research Group, Ton Duc Thang University, Ho Chi Minh City, 70000, Vietnam;15. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, 70000, Vietnam;1. Synlab Group, Microbiology Service, Quirón Madrid University Hospital, Diego de Velázquez 1, 28223 Pozuelo de Alarcón, Madrid, Spain;2. Veterinary Faculty, Alfonso X El Sabio University, Avenida Universidad 1, 28691 Villanueva de la Cañada, Madrid, Spain;3. Parasitology Service, National Centre for Microbiology, Carlos III Health Institute, Ctra. Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain;4. Livestock Laboratory, Regional Government of Álava, Ctra. de Azua 4, 01520 Vitoria-Gasteiz, Spain;5. European University of Madrid, Tajo s/n, 28670 Villaviciosa de Odón, Madrid, Spain |
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| Abstract: | Giardia duodenalis is one of the most frequent intestinal parasitic infections in children worldwide. To date, eight main assemblages of G. duodenalis have been described, but only A and B genetic groups are known to infect humans. In Cuba, this parasite has most clinical impact on children. The aim of this investigation was genetic characterization of G. duodenalis isolated from children with giardiasis diagnosed at the Paediatric Hospital “William Soler” between 2010 and 2011, and to compare the genetic results with clinical and epidemiological data. A total of 103 stool samples from 452 children were positive for G. duodenalis and co-infections with other parasites were noted in 5 cases. Assemblage identification was carried out by the amplification of a fragment of the triosephosphate isomerase (tpi) gene. Sub-assemblages of assemblage A (AI and AII) were identified by a nested PCR using the intergenic spacer (IGS) region of ribosomal deoxyribonucleic acid gene as a target. DNA from 90 of 103 (87.4%) samples was successfully amplified by PCR–tpi. The prevalence of assemblages A and B was 40% and 42%, respectively. Infections with both assemblages were reported in 16 cases. No associations between epidemiological information and assemblage was detected, but assemblage B was significantly (P < 0.01) more frequently found in children with diarrhea, flatulence or abdominal pain than assemblage A. Sub-assemblage AII accounted for the majority of cases (86.5%). |
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| Keywords: | Assemblages PCR Children Cuba |
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