Characterization of Vibrio cholerae from 1986 to 2012 in Yunnan Province,southwest China bordering Myanmar |
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| Institution: | 1. Department of Acute Infectious Diseases Control and Prevention, Yunnan Provincial Centre for Disease Control and Prevention, 650022 Kunming, China;2. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 102206 Beijing, China;1. Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, 5 Yushan Road, Qingdao 266003, China;2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Qingdao 266071, China;1. Federal University of Rio Grande do Sul, Brazil;2. Department of Genetics, Federal University of Pernambuco, Brazil;3. Immunogenetics Laboratory, University of Trieste, Italy;4. Federal University of Health Sciences of Porto Alegre, Brazil;5. Hospital de Clínicas de Porto Alegre, Brazil;1. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;2. Centre for Biodiscovery and Molecular Development of Therapeutics, Queensland Tropical Health Alliance, James Cook University, Cairns, Queensland 4878, Australia;3. Department of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Tropical and Infectious Diseases of Poverty, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA;4. Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;5. Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;1. Hospital Ramón y Cajal, Medicina Tropical y Parasitología Clínica, Servicio de Enfermedades Infecciosas, Madrid, Spain;2. Centro de Investigaciones en Microbiología y Parasitología Tropical, CIMPAT, Universidad de los Andes, Cra 1 No. 18A-10, Bogotá, Colombia;3. Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, Madrid, Spain |
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| Abstract: | Vibrio cholerae is an important infectious pathogen causing serious human diarrhea. We analyzed 568 V. cholerae strains isolated from 1986 to 2012 in Yunnan province, southwest China bordering Myanmar. Polymerase chain reactions for detecting virulence genes, antibiotic susceptibility tests and pulse-field gel electrophoresis (PFGE) were performed. The results showed all the strains were El Tor biotype from 1986. The ctxB subunit sequence analysis for all strains have shown that cholera between 1986 and 1995 was associated with mixed infections with El Tor and El Tor variants, while infections after 1996 were all caused by El Tor variant strains. All of the strains were sensitive to aminoglycosides and quinolone antibiotics while resistant to β-lactamase and carbapenem antibiotics increased gradually. 568 V. cholerae were divided into 218 PFGE-NotI patterns, and the isolates before 2001 and after 2011 were separated into two groups according to PFGE results. The strains isolated before 2001 were mainly referred to native cholera in Yunnan, and after 2011 were primarily referred to as imported strains from Myanmar, which showed the variation of V. cholerae in this area. The molecular characteristics of V. cholerae indicated regularity in bacterial variation and evolution in Yunnan province. |
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| Keywords: | El Tor variants Pulse-field gel electrophoresis Imported strains |
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