基底细胞癌Bcl-2、Bax、Bcl-xl蛋白免疫组化表达的定量研究 |
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引用本文: | 邓列华,赵刚,夏潮湧,陆涛,林泽,黄中新. 基底细胞癌Bcl-2、Bax、Bcl-xl蛋白免疫组化表达的定量研究[J]. 中华皮肤科杂志, 2001, 0(3) |
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作者姓名: | 邓列华 赵刚 夏潮湧 陆涛 林泽 黄中新 |
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作者单位: | 暨南大学医学院第一附属医院皮肤科 !510630广州,暨南大学医学院第一附属医院皮肤科 !510630广州,暨南大学医学院组胚教研室,暨南大学医学院第一附属医院皮肤科 !510630广州,暨南大学医学院第一附属医院皮肤科 !510630广州,暨南大学医学院组胚教研室 |
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基金项目: | 暨南大学医学院科研课题项目!(98027) |
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摘 要: | 目的 通过对基底细胞癌 Bcl-2、 Bax、 Bcl-xl蛋白表达的研究,探讨 3种蛋白在基底细胞癌发生、发展中的作用。 方法 按病理亚型将 46例基底细胞癌分为非侵袭组( 21例)和侵袭组( 25例),采用免疫组化 SP法检测 Bcl-2、 Bax、 Bcl-xl蛋白在石蜡切片上的表达,并对组化结果进行图像分析以获得平均光密度和 Bcl-2/Bax的比值。结果 非侵袭性基底细胞癌组 Bcl-2蛋白表达水平和 Bcl-2/Bax的比值高于侵袭性基底细胞癌组, P值分别为 0.005和 0.044;两组间 Bcl-xl、 Bax蛋白表达水平的差异无显著性, P值分别为 0.097和 0.979。结论 Bcl-2、 Bcl-xl、 Bax蛋白可能参与了基底细胞癌的发生、发展。
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关 键 词: | 基底细胞癌 基因 Bcl-2 基因 Bax 基因 Bcl-xl |
Quantitative Analysis of Bcl-2,Bax, Bcl-xl Expression in Basal Cell Carcinoma with Immunohistochemistry |
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Abstract: | Objective To explore the roles of Bcl-2, Bax, Bcl-xl proteins in the carcinogenesis of basal cell carcinoma(BCC). Methods Forty-six BCC cases were divided into 2 groups (21 in non-aggressive BCC group and 26 in aggressive BCC group) according to their pathological features. Bcl-2, Bax and Bcl-xl were evaluated in formalin fixed, paraffin embedded specimens by immunohistochemical methods and analyzed by image-analysis methods to obtain average optical density (AOD) of each protein for each case. The AOD data of 3 proteins and Bcl-2/Bax ratios were then compared between the 2 BCC groups. Results The level of Bcl-2 expression and Bcl-2/Bax ratio of non-aggressive BCC group were higher than those of aggressive group,(P=0.005 and 0.044, respectively). For the expression level of Bcl-xl and Bax, there was no statistically difference between the two groups (P=0.097 and 0.979, respectively). Conclusion Bcl-2, Bax,Bcl-xl proteins might participate in the carcinogenesis and development of BCC. |
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Keywords: | Basal cell carcinoma Genes bcl-2 Genes bcl-x1 Genes bax |
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