Histamine influence on apoptosis in trophoblast cell cultures |
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Authors: | Pyzlak M Szewczyk G Szukiewicz D Szczesniak A |
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Institution: | 1.Department of General and Experimental Pathology, Medical University of Warsaw, Krakowskie Przedmiescie 26/28, 00-927, Warsaw, Poland ;2.Department of Pathology, Witold Orlowski Clinical Hospital, The Medical Centre of Postgraduate Education, Czerniakowska 231, 00-416, Warsaw, Poland ;3.Department of Oncologic Gynecology, M. Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Wawelska 15, 02-034, Warsaw, Poland ;4.First Department of Obstetrics and Gynecology, Medical University of Warsaw, Zwirki i Wigury 61, 02-091, Warsaw, Poland ; |
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Abstract: | Introduction It has been demonstrated that histamine plays an important role in the pathogenesis of preeclampsia. Histamine regulates the process of differentiation of trophoblast cells; it also acts as a growth factor in malignant melanoma cells, and prevents monocytic apoptosis. Trophoblast research has shown that in preeclampsia placentas, trophoblast apoptosis is significantly increased. Aim of the studyThe aim of our study was to demonstrate the influence of histamine on the process of apoptosis in human trophoblast cell cultures. Materials and methodsPlacentas were obtained after vaginal delivery. Tissue samples were excised from placentas and, with the use of modified Kliman’s method, trophoblast cell cultures were established. The cultures were incubated with dexamethasone as an apoptosis inducer 48 hours prior to apoptosis detection assays. Along with dexamethasone, selected cell cultures were incubated with histamine (1 μmol/l) or histamine (1 μmol/l) and terfenadine (from 1 to 5 μmol/l), a H1 receptor antagonist. For apoptotic activity detection, and quantitative analysis, we used an ELISA assay. M30‐Apoptosense ELISA Kit is based on the M30 monoclonal antibody that binds only the caspase‐cleaved cytokeratin 18 formed during apoptosis in trophoblast cells. ResultsOur investigation showed significantly (p < 0.05) increased apoptotic activity in cultures incubated with dexamethasone, histamine and terfenadine (% of reference value, ±SEM): up to 113.1 ± 4.33%. Cell cultures incubated with dexamethasone and histamine only showed significantly lower apoptotic activity 90.2 ± 5.17%. We suggest that histamine may inhibit apoptotic activity in trophoblast cell cultures via H1 receptor. Thus histamine may regulate the process of trophoblast differentiation (via integrin aV‐b3 expression, as we previously suggested), and influence cell turnover in the placenta. |
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